[目的]探讨北陆花青素的抗氧化作用.[方法]分别采用Fenton反应体系、邻苯三酚自氧化体系、亚油酸体系和普鲁士蓝法来测定北陆花青素对OH·和O·的清除作用、对脂质过氧化物的抑制作用及其还原能力进行评价,并以维生素c和葡萄籽花青素作为阳性时照.[结果]北陆花青紊的抗氧化能力高于葡萄籽花青素和维生素C,对OH·和O·具有很强的清除作用,其IC分别为0.345mg/ml和34.28μg/ml,对脂质过氧化物有明显的抑制作用并有较高的还原能力.[结论]北陆花青素是一种潜在的具有抗氧化活性的天然药物.%[ Objective ] To explore antioxidative activity of anthocyanins from Northland. [ Method] Using anthocyanins from grape seeds and vitamin C as positive control, Fenton reacting system, pyrogallol autoxidation system,linoleic acid system and Prussian blue method were respectively employed to determine OH · scavenging activity,O2 - · scavenging activity,inhibitive effect on lipid peroxidation and reducing capacity of anthocyanins from Northland. [ Result] Antioxidative activity of anthocyanins from Northland was higher than that in anthocyanins from grape seeds and vitamin C. Anthocyanins from Northland had strong scavenging activities on OH · and O2 - ,lC50 of which was 0. 345 mg/ml and 34.28 μg/ml ,respectively. Furthermore ,it was also provided with obvious inhibiting activity on lipid peroxidation and reducing capacity. [ Conclusion ] Anthocyanins from Northland may be a potential natural medieine with antioxidative activity.
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