[目的]建立固相萃取-高效液相色谱-荧光检测法测定水中喹诺酮类抗生素含量.[方法]利用4种喹诺酮类抗生素(诺氟沙星、环丙沙星、洛美沙星和恩诺沙星)的标准品建立标准曲线、喹诺酮类抗生素在水中的检出限及回收率;对采集自不同地区、河段以及自来水的水样使用固相萃取法处理,进行高效液相色谱分析,采用荧光法测定样品中4种喹诺酮类抗生素的含量.[结果]这4种喹诺酮类抗生素在水中的检出限为0.083～0.248μg/L;回收率为63.7%-134.1%.各种水样均不同程度栓出这4种喹诺酮类抗生素,总合量为0.045-3.969μg/L.其中,深圳河水样的喹诺酮类抗生素总含量高于污水,而在自来水中均能检出这4种喹诺酮类抗生素.[结论]采用固相萃取-高效液相色谱-荧光同时测定环境水样中喹诺酮类抗生素是有效可行的,并且价格低廉,能够满足日常监测分析要求.%[ Objective ] To develop a solid phase extraction-high performance liquid chromatography -fluorescence method for determination of quinolone antibiotics in water. [ Method ] The standard curves of four quinolones (norfloxacin, ciprofloxacin, lomefloxacin and enrofloxacin)were prepared. The detection limit in water and recovery were determined. The water samples collected from different areas, river and tap water were treated using solid-phase extraction method and analyzed by high performance liquid chromatography. Then the concentration of quinolones antibiotics was determined by fluorescence method. [ Result] The detection limit of quinolone antibiotics in water was 0.083 -0. 248 μg/L, and their recovery was 63.7％ - 134.1％. The four quinolone antibiotics at different levels were detected in various water samples,and the total concentration of quinolones antibiotics was 0.045 -3. 969 μg/L. The total concentration of quinolones antibiotics was higher in the water samples collected from rivers in Shenzhen area than in the sewage samples. The four quinolone antibiotics could be detected in all tap water samples. [ Conclusion] The solid phase extraction-high performance liquid chromatography-fluorescence method is feasible and effective to detect quinolones in water. In addition, this method needs low cost and can meet requirements of daily monitoring and analysis.
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