[目的]克隆水稻OsOle1基因并对其进行遗传转化.[方法]通过RT-PCR方法对OsOle1基因进行克隆,将克隆出的OsOle1基因连接到pCAMBIA 1300上构建其超表达载体,并通过农杆菌介导对水稻愈伤进行了遗传转化.[结果]克隆出的OsOle1基因全长498bp,编码165个氨基酸,成功构建了其超表达栽体Ub::OsOe1-GUS,最终得到了转基因株系.[结论]为OsOle1基因的功能研究奠定了基础.%[Objective] The cloning and transformation of rice OsOUl gene were conducted in the research. [Method] OsOUl gene waa cloned by RT-PCK. The amplified OsOlel was then ligased to pCAMBIA 1300 to construct CUS overexpression vector. Then the Agrobacuritm mediated method was used in rice callus transformation. [Result] The full-length of OsOUl gene was 498 bp and it encoded 189 amino acids. The over-expression vector Ub:: OtOel -CUS was prepared and transgenic pJants were successfully obtained. [ Conclusion ] The Iransgenic lines laid the foundation for the function research of OsOUl.
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