[Objective] The method of rapid, effective and a large amount of phycoerythrin extracted from Porphyra haitanensis was experi mented. [ Method ] The indicator of the yield and purity of phycoerythrin from the Porphyra haitanensis and the extracting time in three kinds of the extraction techniques: swelling, chemical permeabilization and cutting while stirring were compared. [ Result ] The result showed that the yield of phycoerythrin from the Porphyra haitanensis was 9.71 mg/g, which purity was 0.45 , with swelling technique under the condition of the concentration of Tris-HCl; 0. 01 mol/L, the pH value: 8. 0 and the extracting time: 9 hours; the yield of phycoerythrin was 24. 35 mg/g, which purity was about 0. 22, with chemical permeabilization under the condition of the concentration of SDS: 0. 100 mol/L and the action time: 4 hours and the yield of phycoerythrin was 23. 11 mg/g, which purity was 0. 30, with the cutting while stirring method under the condition of the stirring rate: 18 000 r/min and the working time: 6 minutes. [Conclusion] Based on the integrated comparison, the the cutting while stirring method was best in the extracting efficiency of phycoerythrin including its yield and purity and also a large amount of raw material could be treated at same time, which was an effective method.%[目的]快速、有效、大批量地提取藻红蛋白.[方法]以坛紫菜为原料,采用溶胀法、化学试剂法和搅切法3种粗提技术,以藻红蛋白的提取得率、纯度和提取时间为参数对3种方法进行比较.[结果]溶胀法采用0.01 mol/L Tris-HCl(pH 8.0)浸提9h,藻红蛋白得率为9.71 mg/g,纯度(A565/A280)为0.45;化学试剂法在0.100 mol/L SDS溶液作用4h时,藻红蛋白得率为24.35 mg/g,纯度(A565/A280)为0.22左右;搅切法采用搅切速度18 000 r/min作用6 min,藻红蛋白得率达到23.11 mg/g,纯度(A565/A280)为0.30.[结论]综合比较,搅切法提取藻红蛋白效率高,藻红蛋白得率和纯度较好,处理量较大,是一种十分有效的粗提方法.
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