[Objective] The study aimed to discuss the action of glutathione sulfur of Lotus corniculatu for shifting the enzyme gene LcGST. [ Method] The LcGST was connected to the middle expression vector pGN through the DNA recombination technology. [ Result] The plant expression vector pGN-LcGST was constructed successfully. [ Conclusion] The acquisition of the pGN-LcGST vector laid a foundation for further study on its function in the plant with different source.%[目的]探讨百脉根谷胱甘肽硫转移酶基因(LcGST)的作用.[方法]通过DNA重组技术将LcGST与中间表达载体pGN连接.[结果]成功构建了植物表达载体pGN-LcGST.[结论]pGN-LcGST载体的获得为进一步研究其在异源植物中的功能奠定了基础.
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