[Objective] The aim was to research isolate and identification of the cold resistant degradative strain PTA and its degradative ability. [ Method] Using enrichment culture method, the liquid chromatography and spectrophotometric analysis were used to analyse the degradative ability. [ Result] The train PTA201 was isolated which could degrade PTA and use it as the sole carbon source. Through physiological and biochemical tests and 16S rDNA genetics] analysis, PTA201 belonged to Pseudomonas putida. The degradative rate for PTA at 6 ℃ ,500mg/L was above 99. 8% within 72 hours. The best conditions were 30 ℃, pH 7.0, with inoculating quantity 1% . [ Conclusion] The strain PTA201 could degrade PTA completely in 96 hours under the optimum conditions and it was cold-degrading bacteria..%[目的]研究高效耐冷精对苯二甲酸(PTA)降解菌的分离鉴定及其降解特性.[方法]采用富集培养法,采用液相色谱法和分光光度法分析菌株的降解PTA的性能.[结果]分离出一株能以PTA为唯一碳源生长的耐冷菌菌株PTA201,经16S rDNA基因序列分析,该菌被鉴定为恶臭假单胞菌(Pseudomonas putida).PTA201在6℃,72h内对500 mg/L的PTA降解率达99.8％以上.PTA201降解PTA的最适条件为pH 7.0,温度30℃,接种量1％.[结论]菌株PTA201在最适条件下96h内可以完全降解PTA,是耐冷高效降解菌.
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