[目的]优选马齿苋中抗氧化物质的最佳提取条件.[方法]在单因素试验基础上,选择乙醇浓度、料液比、提取时间和提取次数为影响因素,以1,1-二苯基-2-苦基苯肼(DPPH)自由基清除率为响应值,根据中心组合(Box- Benhnken)试验设计原理,采用4因素3水平的响应面分析法对马齿苋抗氧化活性物质的超声提取工艺进行研究.[结果]在分析各因素显著性及其交互作用的基础上,确定其最佳提取条件为乙醇浓度68％,料液比1:22 (g/ml),提取时间44min,提取次数3次;在此条件下,DPPH自由基清除率为0.911 7,与理论值0.913 5接近.[结论]超声提取法具有提取率高、时间短、超声功率低等优点,可作为马齿苋中抗氧化物质的提取方法.%[Objective] To study the optimum extraction technology of antioxidants from Portulaca oleracea L. [ Method] On the basis of single-factor experiment,the effects of ethanol concentration,liquid-solid ratio,extraction time and extract times on scavenging rate of DPPH radical were inspected. According to the experimental design principles of Box-Benhnken,a four-factor and three-level response surface modeling (RSM) was adopted to study the ultrasonic extraction technology of antioxidant from Portulaca oleracea L. [ Result] Based on analyzing the significance of each factor and their interactions,the optimum extraction conditions were determined as follows:the ethanol concentration was 68% ,the solid-liquid ratio was 1:22, the extraction time was 44 min and the extraction times was 3. Under the optimized conditions, the actual scavenging rate of DPPH free radical was 0.911 7,close to the predicted value. [Conclusion]The ultrasonic method had the advantages of high extraction rate,short time and low ultrasonic power,therefore it could be the method for extracting antioxidant compounds from Portulaca oleracea L.
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