[Objective] The aim of this study was to isolate and identify lactic acid bacteria ( LAB) from wheat haulm and to select efficient strains for silage fermentation. [ Method] From 78 LAB strains isolated on MRS + CaCO3 solid medium,we selected 43 strains having better acid-production ability through morphological observation, Gram staining, physiological and biochemical tests, acid production test, acid tolerance test and salt tolerance test. These strains were finally identified by sequencing 16S rDNA. [ Result] Of the 43 LAB strains having better acid-production ability ,37 were Lactobacillus paracasei subsp. ,one was Lactobacillus rhamnosusand five were Enterococcus faecium ,as shown by sequences of 16S rDNA. [Conclusion] A total of 43 LAB strains having better acid-production ability were selected,which may be developed as high-quality silage additives.%[目的]从小麦秸秆发酵液中筛选并鉴定适合于青贮添加剂的高效乳酸菌.[方法]用MRS+ CaCO3固体培养基筛选,分离出78株乳酸菌；经形态学、革兰氏染色、生理生化试验、产酸效率试验、耐酸能力试验和耐盐试验等挑选出43株产酸率高的乳酸菌.通过16S rDNA法对该乳酸菌进行鉴定.[结果]已获得43株产酸能力强的菌株,16S rDNA鉴定结果表明,37株为副干酪乳杆菌亚种(Lactobacillus paracasei subsp.),1株为鼠李糖乳杆菌(Lactobacillus rhamnosus),5株为肠球菌(Enterococcus faecium).[结论]从小麦秸秆中筛选出43株具有较强产酸能力的乳酸菌,对研发优质青贮饲料添加剂具有一定的指导意义.
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