[Objective] The purpose of the study was to supply technical support for the rapid qualitative analysis on Chinese medicine rheum. [Method] The rheum aqueous extracts with different concn. were prepared with Qinghai rheum as tested material and the fluorescence scanning was performed on the aqueous extracts. The fluorescence scanning was performed again after HOAc-NaOAc buffer solutions with different pH values were added into the selected rheum aqueous extracts with certain concn. [Result] As the concn. of rheum aqueous extracts was reduced, the fluorescence intensity was reduced gradually, its fluorescence intensity showed a linear relationship with its concn. When the pH value of buffer solution was 2.6-4.2, the fluorescence intensity was increased as the pH value was increased. When the pH value of buffer solution was 4.2-5.8, the fluorescence intensity was reduced as the pH value was increased. When the pH value of buffer solution was 4.8-5.2, the fluorescence intensity was nearly invariable. There was an obvious fluorescence peak in the three-dimensional fluorescence contour spectrogram of the aqueous extracts of Chinese medicine rheum and its emission wavelength was at 401.8 nm. [Conclusion] The condition of determining the aqueous extracts of Chinese medicine rheum by fluorescence spectrum analysis was ascertained in this experiment. It provided a new method for the qualitative analysis on Chinese medicine rheum.%[目的] 为中药大黄的快速定性分析提供技术支持.[方法] 以青海大黄为试材,制备不同浓度的大黄水浸液并对其进行荧光扫描;取一定浓度的大黄水浸液,加入不同pH值的HOAc-NaOAc缓冲溶液后对其进行荧光扫描.[结果] 随着大黄水浸液浓度的减小,荧光强度逐渐减小,其荧光强度与浓度呈线性关系.当缓冲溶液的pH值为2.6～4.2时,荧光强度随着pH值的增大而增大.当缓冲溶液的pH值为4.2～5.8时,荧光强度随着pH值的增大而减小.当缓冲溶液的pH值为4.8～5.2时,荧光强度几乎不变.中药大黄水浸液的三维荧光等高线光谱图中有一个明显的荧光峰,其发射波长位于401.8 nm.[结论] 该试验确定了中药大黄水浸液荧光光谱分析法的测定条件,为中药大黄的定性分析提供了新方法.
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