[Objective] The theoretical reference for the large population of pteridophyte multiplication was provided through the study on the most suitable sterilization method and best concentration of gibberellin acid for its seedling growth. [ Method] The pteridophyte seedling growth under different sterilization ways and concentrations of gibberellin acid was studied. [Results] The results showed that the longer the disinfection of alcohol and mercuric chloride was, the better the effect of its sterilization was and the more its poisoning effect on young seedling was. The average poisoning rate was increased from 14.47 to % to 25.57% when the disinfection time of mercuric chloride was prolonged from 8 minutes to 12 minutes. The rate of callus induction under the treatment of mercuric chloride was higher than that of other two kinds of processing. The rate of pteridophyte seedling formation in the medium added with gibberellin acid was significantly improved after t test(t =3.76 >t_(0.05) =3. 18). Within a certain extent, the rate of pteridophyte seedling formation was increased with the increment of GA concentration, and it was decreased if beyond the extent, however, the rate of callus induction was increased gradually. [Conclusion] It can be seen that the best efficiency on pteridophyte seedling growth was from the treatment of the washing with tap water for 2 hours, the disinfectant with alcohol for 60 s, and then with mercuric chloride for 10 minutes. The best concentration of gibberellin acid added in medium for pteridophyte seedling growth was 1.5 mg/L.%[目的]研究最适合凤丫蕨幼茎直接成苗的灭菌手段和赤霉素浓度,为其大量繁殖提供理论参考.[方法]在不同灭菌方法和赤霉素浓度下,对凤丫蕨进行幼茎成苗研究.[结果]结果显示,酒精和升汞消毒时间越长,灭菌效果越好,但对幼茎的毒害作用也越强,升汞处理时间由8 min改为12 min时,平均毒害率由14.47%上升为25.57%,而愈伤诱导率比其他2种处理要高;在添加赤霉素的培养基上幼茎成苗率明显较高,t测验差异达到0.05显著水平(t=3.76> t_(0.05)=3.18).在一定范围内,随着赤霉素浓度的增加,幼茎成苗率呈现上升趋势;但随着浓度的进一步增大,成苗率反而下降,而愈伤组织的诱导率逐渐上升.[结论]用自来水洗2 h,酒精消毒60 s,升汞消毒10 min对幼茎的灭菌效果好,且对幼茎毒害小;最适合诱导幼茎直接成苗的赤霉素浓度为1.5 mg/L.
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