[Objective] The research aimed to supply an accurate, intelligent and automatic micro-analytical method for the quality identification and genetic analysis on rice. [Method] The amylose content in rice was determined by national standard method and flow-analysis with UV-visible spectrophotometer and full-automatic continuous flowing analyzer resp. [Result] In the stability test on samples, the average standard deviation of dispersed liquids of the 5 rice samples was 0.000 5 and their average variation coefficient was 0.34%. When the analytical velocity was 25-50 samples/h, the variation coefficient among repeats was relatively stable. When the analytical velocity was 25-45 samples/h, both peak shape and separation effect were better. In precision test, the average standard deviation of the absorbance of sample solutions was 0.000 4 and their average variation coefficient was 0.20%. The average standard deviation of determination results of the 2 methods was 0.107 7 and their average variation coefficient was 0.61%. The average standard deviation and average variation coefficient of flow-analysis were 0.082 1 and 0.49% resp. and that of national standard method were 0.111 9 and 0.64% resp. [Conclusion] This method was relatively perfect for determining the amylose content in rice.%[目的]为稻米品质鉴定及遗传分析提供一种精确的、智能化、自动化的微量分析方法.[方法]应用紫外可见分光光度计和全自动连续流动分析仪,分别采用国标法和流动分析法测定了稻米中直链淀粉的含量.[结果]在样品稳定性试验中,5个稻米样品分散液的平均标准差为0.000 5,平均变异系数为0.34%.当分析速度为25～50 样/h时,重复间变异系数比较稳定;当分析速度为25～45 样/h时,峰形和分离效果均较好.在精确度试验中,样品液吸光度的平均标准差为0.000 4,平均变异系数为0.20%.比较两种方法的测定结果的重现性,用5个品种,每个品种10次重复测定,其平均标准差为0.1077,平均变异系数为0.61%.流动分析法的平均标准偏差和平均变异系数分别为0.082 1和0.49%,国标法的分别为0.111 9和0.64%.[结论]该方法对于测定稻米直链淀粉含量是较为理想的.
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