[Objective] To establish suspension culture system of cell embryogenesis and fast seedling formation of Xanthoceras sorbifolia , and provide reference for the developments of industrialization and scale. [Method] Taking new and old seed of Xanthoceras sorbifolia as material, inducing embryogenesis, the cell suspension culture of Xanthoceras sorbifolia was carried out. [Result] The optimal medium of MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+2,4-D 0.5 mg/L+3% sucrose was suitable for embryogenic callus induction. The optimal medium of MS+6-BA 1.0 mg/L+NAA 1.0 mg/L+3% sucrose was suitable for adventitious buds induction culture of embryogenic callus, germination rate was highest. The optimal medium of MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+ 3% sucrose was suitable for regenerated plant culture of single cell mass. [Conclusion] NAA mixed with 2,4-D was more beneficial to the formation of embryogenic callus. NAA has large promoting effect on callus germination culture of Xanthoceras sorbifolia at some concentration.%[目的]建立文冠果体细胞胚胎发生和快速成苗的悬浮培养体系,为文冠果的产业化和规模化发展提供参考.[方法]以文冠果新旧种子为材料,诱导体胚发生,进行文冠果细胞悬浮培养.[结果]适合胚性愈伤诱导的最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+2,4-D 0.5 mg/L+3%蔗糖;适合胚性愈伤组织不定芽诱导培养的最佳诱导培养基为MS+6-BA 1.0 mg/L+NAA 1.0 mg/L+3%蔗糖,且萌芽率最高;适合单细胞团再生植株培养的最佳培养基为MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+3%蔗糖.[结论]NAA和2,4-D混合使用有利于胚性愈伤组织的形成;一定浓度的NAA对文冠果愈伤组织萌芽培养有较大的促进作用.
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