[目的]提取肉苁蓉多糖并测定其抑菌作用.[方法]采用热水浸提法提取肉苁蓉多糖,在单因素试验的基础上,通过3因素3水平正交试验优化肉苁蓉多糖提取工艺.[结果]各因素对多糖得率的影响依次为浸提温度＞水料比＞浸提时间.最佳提取工艺为以1:15的料水比在85 ℃下浸提2 h,利用该工艺提取肉苁蓉多糖的平均得率为6.785 7 mg/g.肉苁蓉多糖溶液对四叠球菌的抑制作用最强,其抑菌圈直径为11.20 mm,其次为枯草芽孢杆菌,其抑菌圈直径为10.24 mm.肉苁蓉多糖溶液对啤酒酵母的抑制作用较强,其抑菌圈直径为8.16 mm,对黑曲霉和米曲霉的抑菌作用不明显.肉苁蓉多糖溶液对大肠杆菌、四叠球菌、枯草杆菌、啤酒酵母和橘青霉的最小抑菌浓度分别为0.437、0.109、0.218、0.437和0.874 mg/ml.[结论]该研究为肉苁蓉在更多领域中的合理开发和利用奠定了基础.%[Objective]The purpose was to extract Cistanche deserticola polysaccharides and determine their antimicrobial action. [Method] The polysaccharides were extracted from C. deserticola by hot water extraction. On the basis of single factor experiments, the extraction technology was optimized through 3-factor and 3-level orthogonal test. [Result]The influences of various factors on the polysaccharide yield were as follows: extraction temperature > ratio of water to material > extraction time. The optimum extraction technology was extraction with ratio of material to water of 1:15 at 85 ℃ for 2 h. The average yield rate of polysaccharides extracted from C. deserticola by this technology was 6.785 7 mg/g. The inhibitory action of C. deserticola polysaccharides solution on Micrococcus terragenus was strongest and its antibacterial circle diameter was 11.20 mm; that on Bacillus subtilis was secondary and its antibacterial circle diameter was 10.24 mm. The inhibitory action of C. deserticola polysaccharides solution on Scerevisiae hensen was stronger and its antibacterial circle diameter was 8.16 mm and that on Aspergillus nigers and Aspergillus oryzae was not obvious. The minimum inhibitory concentrations of C. deserticola polysaccharides solution on Escherichia coli , M. terragenus, B. subtilis, S. hensen and Penicillium sp. were 0.437, 0.109, 0.218, 0.437 and 0.874 mg/ml resp. [Conclusion]This study laid a foundation for the reasonable development and utilization of C. deserticola in more domains.
展开▼
