[Objective] The aim was to supply technical support for solving the problem that the sewage was difficult to dispose in north China in winter. [Method] The influences of pH value, temperature and time on the dehydrogenase activity in 8 strains of cold-resistant bacteria isolated, purified and identified from low-temperature biofilm were analyzed by TTC method. [Result] The optimum pH value of the strains S1, S4, S5, S7 and S8 at both optimum temperature and 4 ℃ was 7.5 and that of the other 3 strains at the 2 temperatures were different, but they were in the range of 7.5-8.5. The optimum reaction temperature of the strains S1, S3, S4, S5 and S6 was 30 ℃ and that of the strains S2, S7 and S8 was 20 ℃. Except for the strain S3, the optimum reaction time of the other strains at 4 ℃ was longer than their optimum reaction time at optimum reaction temperature. At 4 ℃, the optimum pH values of the strains S3, S4 and S6 were 8.5, 7.5 and 8.0 resp., their optimum reaction time were 0.6, 12.0 and 0.5 h resp. and their highest dehydrogenase activities were 7.19, 6.73 and 7.70 mg(TF)/g(SS) resp. [Conclusion] The dehydrogenase activities of the strains S3, S4 and S6 were higher and they were suitable to be used in processing low-temperature sewage.%[目的]为解决我国北方冬季污水处理困难的问题提供技术支持.[方法]运用TTC法分析了pH值、温度和时间对从低温生物膜中分离纯化鉴定得到8株耐冷菌中脱氢酶活性的影响.[结果]菌株S1、S4、S5、S7和S8在最适温度和4 ℃下的最适pH值都是7.5,其他3株菌在2种温度下的最适pH值不同,但都在7.5～8.5的范围内.菌株S1、S3、S4、S5和S6的最适反应温度为30 ℃,菌株S2、S7和S8的最适反应温度为20 ℃.除菌株S3外,其余菌株在4 ℃下的最适反应时间比最适反应温度下的长.在4 ℃下,菌株S3、S4和S6的最适pH值和最适反应时间分别为8.5和0.6 h、7.5和12.0 h、8.0和0.5 h,其最高脱氢酶活性分别为7.19、6.73和7.70 mg(TF)/g(SS).[结论]菌株S3、S4和S6的脱氢酶活性较高,适合用于处理低温污水.
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