[目的]研究中华桫椤总DNA的提取方法.[方法]采用SDS法和改良CTAB法提取中华桫椤总DNA,通过电泳检测方法进行比较.[结果]用改良CTAB法得到的DNA浓度和纯度都较高,基本上无蛋白质、多糖等杂质.用改良CTAB法得到的总DNA进行ISSR-PCR扩增,谱带清晰,获得较好的效果.[结论]为提取高质量的DNA样品奠定了基础,并为探讨中华桫椤自然居群的遗传多样性和种群遗传结构及进一步保护和利用中华桫椤提供了分子遗传学依据.%[Objective] The research aimed to study the total DNA extraction methods of A. costularis Baker.[Method] To investigate the optimum DNA extraction method, SDS and modified CTAB methods for isolating genomic DNA from A. costularis Baker leaf were studied. The isolated DNA was examined by ultraviolet absorption test and agarose gelelectrophoresis. [Result] The results showed that the modified CTAB method was suitable for DNA isolation from A. costularis Baker plants. The quality of DNA could meet ISSR requirement even extracted once by the modified CTAB method. [Conclusion] The study can provide the basis for extracting high quality DNA sample, and can offer the molecular genetics reference for exploring the genetic diversity and the population genetic structure of A. costularis Baker and deeply protecting and utilizing A. costularis Baker.
展开▼
