Comprehensive evaluation of the metabolic effects of porcine CRTC3 overexpression on subcutaneous adipocytes with metabolomic and transcriptomic analyses

Jiaqi Liu; Jie Li; Wentao Chen; Xintao Xie; Xingang Chu; Teresa G.Valencak; Yizhen Wang; Tizhong Shan

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Comprehensive evaluation of the metabolic effects of porcine CRTC3 overexpression on subcutaneous adipocytes with metabolomic and transcriptomic analyses

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Background:Meat quality is largely driven by fat deposition,which is regulated by several genes and signaling pathways.The cyclic adenosine monophosphate(cAMP)-regulated transcriptional coactivator 3(CRTC3)is a coactivator of cAMP response element binding protein(CREB)that mediates the function of protein kinase A(PKA)signaling pathway and is involved in various biological processes including lipid and energy metabolism.However,the effects of CRTC3 on the metabolome and transcriptome of porcine subcutaneous adipocytes have not been studied yet.Here,we tested whether porcine CRTC3 expression would be related to fat deposition in Heigai pigs(a local fatty breed in China)and Duroc×Landrace×Yorkshire(DLY,a lean breed)pigs in vivo.The effects of adenovirus-induced CRTC3 overexpression on the metabolomic and transcriptomic profiles of subcutaneous adipocytes were also determined in vitro by performing mass spectrometry-based metabolomics combined with RNA sequencing(RNA-seq).Results:Porcine CRTC3 expression is associated with fat deposition in vivo.In addition,CRTC3 overexpression increased lipid accumulation and the expression of mature adipocyte-related genes in cultured porcine subcutaneous adipocytes.According to the metabolomic analysis,CRTC3 overexpression induced significant changes in adipocyte lipid,amino acid and nucleotide metabolites in vitro.The RNA-seq analysis suggested that CRTC3 overexpression alters the expression of genes and pathways involved in adipogenesis,fatty acid metabolism and glycerophospholipid metabolism in vitro.Conclusions:We identified significant alterations in the metabolite composition and the expression of genes and pathways involved in lipid metabolism in CRTC3-overexpressing adipocytes.Our results suggest that CRTC3 might play an important regulatory role in lipid metabolism and thus affects lipid accumulation in porcine subcutaneous adipocytes.

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《畜牧与生物技术杂志：英文版》 |2021年第3期|P.891-903|共13页
作者
Jiaqi Liu; Jie Li; Wentao Chen; Xintao Xie; Xingang Chu; Teresa G.Valencak; Yizhen Wang; Tizhong Shan;
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College of Animal Sciences Zhejiang University Hangzhou ChinaKey Laboratory of Molecular Animal Nutrition(Zhejiang University) Ministry of Education Hangzhou ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province 866 Yuhangtang Road Hangzhou China;

College of Animal Sciences Zhejiang University Hangzhou ChinaKey Laboratory of Molecular Animal Nutrition(Zhejiang University) Ministry of Education Hangzhou ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province 866 Yuhangtang Road Hangzhou China;

College of Animal Sciences Zhejiang University Hangzhou ChinaKey Laboratory of Molecular Animal Nutrition(Zhejiang University) Ministry of Education Hangzhou ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province 866 Yuhangtang Road Hangzhou China;

College of Animal Sciences Zhejiang University Hangzhou China;

College of Animal Sciences Zhejiang University Hangzhou China;

College of Animal Sciences Zhejiang University Hangzhou China;

College of Animal Sciences Zhejiang University Hangzhou ChinaKey Laboratory of Molecular Animal Nutrition(Zhejiang University) Ministry of Education Hangzhou ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province 866 Yuhangtang Road Hangzhou China;

College of Animal Sciences Zhejiang University Hangzhou ChinaKey Laboratory of Molecular Animal Nutrition(Zhejiang University) Ministry of Education Hangzhou ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province 866 Yuhangtang Road Hangzhou China;

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正文语种 chi
中图分类家畜;
关键词
CRTC3; Energy metabolism; Metabolomics; Overexpression; Subcutaneous adipocytes; Transcriptomics;

Comprehensive evaluation of the metabolic effects of porcine CRTC3 overexpression on subcutaneous adipocytes with metabolomic and transcriptomic analyses

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