Examination of the molecular control of ruminal epithelial function in response to dietary restriction and subsequent compensatory growth in cattle

Emma OShea; Sinéad M.Waters; Kate Keogh; Alan K.Kelly; David A.Kenny

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Examination of the molecular control of ruminal epithelial function in response to dietary restriction and subsequent compensatory growth in cattle

机译：响应饮食限制和随后的牛代偿性生长对瘤胃上皮功能的分子控制的检查

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Background:The objective of this study was to investigate the effect of dietary restriction and subsequent compensatory growth on the relative expression of genes involved in volatile fatty acid transport,metabolism and cell proliferation in ruminal epithelial tissue of beef cattle.Sixty Holstein Friesian bulls (mean liveweight 370 ± 35 kg;mean age 479 ± 15 d) were assigned to one of two groups:(i) restricted feed allowance (RES;n =30) for 125 d (Period 1) followed by ad libitum access to feed for 55 d (Period 2) or (ii) ad libitum access to feed throughout (ADLIB;n =30).Target growth rate for RES was 0.6 kg/d during Period 1.At the end of each dietary period,15 animals from each treatment group were slaughtered and ruminal epithelial tissue and liquid digesta harvested from the ventral sac of the rumen.Real-time qPCR was used to quantify mRNA transcripts of 26 genes associated with ruminal epithelial function.Volatile fatty acid analysis of rumen fluid from individual animals was conducted using gas chromatography.Results:Diet × period interactions were evident for genes involved in ketogenesis (BDH2,P =0.017),pyruvate metabolism (LDHa,P=0.048;PDHA 1,P =0.015) and cellular transport and structure (DSG1,P=0.019;CACT,P=0.027).Ruminal concentrations of propionic acid (P =0.018) and n-valeric acid (P =0.029) were lower in RES animals,compared with ADLIB,throughout the experiment.There was also a strong tendency (P=0.064) toward a diet × period interaction for n-butyric with higher concentrations in RES animals,compared with ADLIB,during Period 1.Conclusions:These data suggest that following nutrient restriction,the structural integrity of the rumen wall is compromised and there is upregulation of genes involved in the production of ketone bodies and breakdown of pyruvate for cellular energy.These results provide an insight into the potential molecular mechanisms regulating ruminal epithelial absorptive metabolism and growth following nutrient restriction and subsequent compensatory growth.

机译：背景：本研究的目的是研究饮食限制和随后的代偿性生长对肉牛瘤胃上皮组织中挥发性脂肪酸转运，代谢和细胞增殖相关基因的相对表达的影响。体重370±35公斤;平均年龄479±15 d）分为以下两组之一：（i）125 d（时段1）的限制饲料允许量（RES; n = 30），然后自由采食55 d（第2阶段）或（ii）整个过程中自由采食（ADLIB; n = 30）.RES的目标生长率在第1阶段为0.6 kg / d。在每个饮食阶段结束时，每种处理15只动物屠宰组，从瘤胃腹囊中收集瘤胃上皮组织和消化液。采用实时qPCR定量分析与瘤胃上皮功能有关的26个基因的mRNA转录本。结果：与生酮相关的基因（BDH2，P = 0.017），丙酮酸代谢（LDHa，P = 0.048; PDHA 1，P = 0.015），细胞转运和结构（DSG1， P = 0.019; CACT，P = 0.027）。在整个实验过程中，与ADLIB相比，RES动物的丙酸（P = 0.018）和正戊酸（P = 0.029）的浓度较低。阶段1中RES动物中较高浓度的正丁酸与饮食的周期变化趋势（P = 0.064），与ADLIB相比，结论1。结论：这些数据表明，在营养限制后，瘤胃壁的结构完整性受到损害这些结果提供了调控营养物质限制和随后的瘤胃上皮吸收代谢和生长的潜在分子机制的见解。补偿性增长。

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《畜牧与生物技术杂志(英文版)》 |2017年第1期|107-118|共12页
作者
Emma OShea; Sinéad M.Waters; Kate Keogh; Alan K.Kelly; David A.Kenny;
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School of Agriculture and Food Science, University College Dublin, Belfield,Dublin 4, Ireland;

Animal and Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc Grange, Dunsany, Co.Meath, Ireland;

UCD Earth Institute, University College Dublin, Belfield,Dublin 4, Ireland;

Animal and Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc Grange, Dunsany, Co.Meath, Ireland;

Animal and Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc Grange, Dunsany, Co.Meath, Ireland;

School of Agriculture and Food Science, University College Dublin, Belfield,Dublin 4, Ireland;

School of Agriculture and Food Science, University College Dublin, Belfield,Dublin 4, Ireland;

Animal and Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc Grange, Dunsany, Co.Meath, Ireland;

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Examination of the molecular control of ruminal epithelial function in response to dietary restriction and subsequent compensatory growth in cattle

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