Effect of in ovo folic acid injection on hepatic IGF2 expression and embryo growth of broilers

Yanli Liu; Lihui Zhi; Jing Shen; Shizhao Li; Junhu Yao; Xiaojun Yang

摘要

Background:Insulin-like factor 2 (IGF2) plays an important role in embryonic growth process by modulating intermediary metabolism and cell proliferation.Folic acid is involved in one carbon metabolism and contributes to DNA methylation which is related to gene expression.The purpose of this study was to explore whether folic acid could regulate IGF2 expression via epigenetic mechanism and further promote embryonic growth of new-hatched broilers.Methods:In the present study,360 fertile eggs were selected and randomly assigned to four treatments.On 11 embryonic day of incubation (E11),0,50,100 and 150 μg folic acid were injected into eggs respectively.After hatched,growth performance of broilers were calculated.Hepatic IGF2 expression,methylation level and chromatin structure of promoter region were analyzed.Results:Results have showed that IGF2 expression was up-regulated in 150 μg folic acid group (P ＜ 0.05) and other two dose of folic acid did not affect gene expression (P ＞ 0.05).Meanwhile,methylation level of IGF2 promoter were lower in 100 and 150 μg groups,which was consistent with lower expression of DNA methyltransferase 1 (DNMT1) (P ＜ 0.05).What's more,chromatin looseness of IGF2 promoter was higher in 150 μg group than control group (P＜ 0.05).Further,birth weight (BW),liver and bursa index of new-hatched chickens in 150 μg folic acid group were higher than the other groups (P ＜ 0.05).There were positive correlations between hepatic IGF2 expression and BW and organs index (P ＜ 0.05).Conclusion:In conclusion,our data have demonstrated that 150 μg folic acid injection on E11 could up-regulate IGF2 expression by modulating DNA hypomethylation and improving chromatin accessibility in the gene promoter region,and ulteriorly facilitate embryonic growth and organ development of broilers.

机译：背景：胰岛素样因子2（IGF2）通过调节中间代谢和细胞增殖在胚胎生长过程中发挥重要作用。叶酸参与一种碳代谢并促进与基因表达有关的DNA甲基化。本研究旨在探讨叶酸是否可以通过表观遗传机制调节IGF2的表达，并进一步促进新孵化肉鸡的胚胎生长。方法：本研究选择了360个可育卵，并随机分为4种处理方法。在孵化的第11个胚胎天（E11）分别向卵中注入0,50,100和150μg叶酸，孵化后计算肉鸡的生长性能，分析肝脏IGF2的表达，启动子区域的甲基化水平和染色质结构。结果：结果表明，IGF2在150μg叶酸组中表达上调（P ＜0.05），另外两剂叶酸均不影响基因表达（P＞ 0.05）。同时，IGF2启动子的甲基化水平在100和150μg组较低，这与DNA甲基转移酶1（DNMT1）的表达较低有关（P ＜0.05）。此外，IGF2启动子的染色质疏松度在150μg组高于对照组（P ＜0.05）。叶酸组150g新孵出的雏鸡的进一步，出生体重（BW），肝脏和法氏囊指数均高于其他组（P ＜0.05）。肝脏IGF2之间呈正相关结论：结论是，我们的数据表明，在E11上注射150μg叶酸可以通过调节DNA低甲基化并改善基因启动子区域的染色质可及性来上调IGF2的表达，并且有利于肉鸡的胚胎生长和器官发育。

Effect of in ovo folic acid injection on hepatic IGF2 expression and embryo growth of broilers

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