Determination of basal ileal endogenous losses and standardized ileal digestibility of amino acids in barley fed to growing pigs

Hanna Katharina Spindler; Rainer Mosenthin; Pia Rosenfelder; Henry J(φ)rgensen; Knud Erik Bach Knudsen; Meike Eklund

摘要

Background:Basal ileal endogenous amino acid (AA) losses (IAAend) and standardized ileal digestibility (SID) values of cereal grains,such as barley,are apparently underestimated when determined according to the nitrogen (N)-free method.Regression analysis between the dietary apparent ileal digestible content (cAID) and total crude protein (CP) and AA can be considered as alternative approach to obtain more accurate values for IAAend and SID of AA in cereal grains.Methods:Eight hulled barley genotypes were used,with barley being the only source of CP and AA in the assay diets.The diets contained 95 ％ as-fed of these eight barley genotypes each,ranging in CP content between 109.1 and 123.8 g/kg dry matter (DM).Nine ileally T-cannulated barrows,average body weight (BW) 30± 2 kg,were allotted to a row-column design comprising eight periods with 6 d each and nine pigs.On d 5 and the night of d 6 of every period,ileal digesta were collected for a total of 12 h.The IAAend and the SID were determined by linear regression analysis between cAID and total dietary CP and AA.Results:There exist linear relationships between cAID and total CP and AA (P ＜ 0.001).The IAAend of CP,Lys,Met,Thr and Trp amounted to 35.34,1.08,0.25,1.02 and 0.38 g/kg DM intake (DMI),respectively,which are greater compared to average IAAend determined previously under N-free feeding conditions.The SID of CP,Lys,Met,Thr and Trp was 90,79,85,79 and 86 ％,respectively,and was greater when compared to tabulated values.Moreover,these SID values were greater than those reported in literature,based on correction of apparent ileal digestibility (AID) of CP and AA for their IAAend values.Summarized,the results of the present regression analysis indicate greater IAAend in barley-based diets compared to those obtained by N-free feeding.Conclusions:For low-protein feed ingredients like barley the regression method may be preferred over correction of AID values for their IAAend determined under N-free feeding conditions,as intercepts and slopes of the linear regression equations between cAID and total dietary CP and AA provide direct estimates of IAAend and SID of CP and AA in the presence of the assay feed ingredient.

机译：背景：根据无氮法确定谷物的谷类回肠内源氨基酸（AA）损失（IAAend）和标准化回肠消化率（SID）值显然被低估了。日粮的表观回肠消化率（cAID）和总粗蛋白（CP）和AA可以被认为是获得谷物中AA的IAAend和SID的更准确值的替代方法。方法：使用八种去壳大麦基因型，其中大麦为在日粮中，CP和AA的唯一来源。日粮中这8种大麦基因型的日粮含量均为95％，其CP含量介于109.1至123.8 g / kg干物质（DM）之间。将平均体重（BW）30±2 kg的猪按每列6天和9头猪的八个时期分配给行列设计。在每个时期的第5天和第6天晚上，收集回肠消化总共12个小时。结果：cAID与总CP和AA之间存在线性关系（P ＜0.001）。CP，Lys，Met，Thr和Trp的IAAend分别为35.34、1.08。分别为0.25、1.02和0.38 g / kg DM摄入量（DMI），高于先前在无氮饲喂条件下确定的平均IAAend。CP，Lys，Met，Thr和Trp的SID为90,79，分别为85,79和86％，与列表值相比更大。基于对CP和AA的IAAend值的表观回肠消化率（AID）的校正，这些SID值大于文献报道的SID。综上所述，本回归分析的结果表明，与通过无氮喂养获得的日粮相比，基于大麦的日粮中的IAAend更高。结论：对于大麦等低蛋白饲料成分，回归方法可能优于校正AID值的方法无氮饲喂条件下确定的IAAend s，因为在存在分析饲料成分的情况下，cAID与日粮总CP和AA之间线性回归方程的截距和斜率可以直接估算CP和AA的IAAend和SID。

Determination of basal ileal endogenous losses and standardized ileal digestibility of amino acids in barley fed to growing pigs

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