Expression of genes involved in progesterone receptor paracrine signaling and their effect on litter size in pigs

Xiao Chen; Jinluan Fu; Aiguo Wang

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Expression of genes involved in progesterone receptor paracrine signaling and their effect on litter size in pigs

机译：孕激素受体旁分泌信号转导相关基因的表达及其对猪产仔数的影响

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Background:Embryonic morality during the period of implantation strongly affects litter size in pigs.Progesterone receptor (PGR) paracrine signaling has been recognized to play a significant role in embryonic implantation.IHH,NR2F2,BMP2,FKBP4 and HAND2 were proved to involve in PGR paracrine signaling.The objective of this study was to evaluate the expression of IHH,NR2F2,BMP2,FKBP4 and HAND2 in endometrium of pregnant sows and to further investigate these genes' effect on litter size in pigs.Real-time PCR,western blot and immunostaining were used to study target genes/proteins expression in endometrium in pigs.RFLP-PCR was used to detect single nucleotide polymorphisms (SNPs) of target genes.Results:The results showed that the mRNA and protein expression levels of IHH,NR2F2 and BMP2 were up-regulated during implantation period (P ＜ 0.05 or P ＜ 0.01).All target proteins were mainly observed in luminal epithelium and glandular epithelium.Interestingly,the staining of NR2F2 and HAND2 was also strong in stroma.SNPs detection revealed that there was a-204C ＞ A mutation in promoter region of NR2F2 gene.Three genotypes were found in Large White,Landrace and Duroc sows.A total of 1847 litter records from 625 sows genotyped at NR2F2 gene were used to analyze the total number born (TNB) and number born alive (NBA).The study of the effect on litter size suggested that sows with genotype CC tend to have higher litter size.Conclusions:These results showed the expression patterns of genes/proteins involved in PGR paracrine signaling over implantation time.And the candidate gene for litter size was identified from genes involved in this signaling.This study could be a resource for further studies to identify the roles of these genes for embryonic implantation in pigs.

机译：背景：胚胎植入期间的胚胎道德状况严重影响猪的产仔数，已认识到孕酮受体（PGR）旁分泌信号在胚胎植入中起重要作用.IHH，NR2F2，BMP2，FKBP4和HAND2被证明与PGR有关本研究的目的是评估妊娠母猪子宫内膜中IHH，NR2F2，BMP2，FKBP4和HAND2的表达，并进一步研究这些基因对猪产仔数的影响。实时PCR，western blot和免疫染色法研究猪子宫内膜中靶基因/蛋白的表达。RFLP-PCR检测靶基因的单核苷酸多态性（SNPs）。结果：结果表明，IHH，NR2F2和BMP2的mRNA和蛋白表达水平在植入期间均被上调（P ＜0.05或P ＜0.01）。所有靶蛋白均主要在腔上皮和腺上皮中观察到。有趣的是，NR2F2和HAND2的染色为SNPs检测发现NR2F2基因启动子区存在a-204C＞ A突变。在大白，长白和杜洛克母猪中发现了3个基因型。625个母猪在NR2F2基因型中共记录了1847个窝。通过对出生总数（TNB）和存活数（NBA）进行分析。对产仔数影响的研究表明，基因型CC的母猪倾向于具有更高的产仔数。结论：这些结果表明了基因的表达方式蛋白质在植入过程中参与了PGR旁分泌信号转导，并从参与该信号转导的基因中鉴定出产仔数的候选基因。该研究可为进一步研究这些基因在猪胚胎植入中的作用提供资源。

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《畜牧与生物技术杂志(英文版)》 |2017年第1期|30-42|共13页
作者
Xiao Chen; Jinluan Fu; Aiguo Wang;
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作者单位

College of Animal Sciences and Technology, National Engineering Laboratory for Animal Breeding & Key Laboratory of Animal Genetics,Breeding and Reproduction, Ministry of Agriculture, China Agricultural University, Beijing 100193, People's Republic of China;

Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, People's Republic of China;

College of Animal Sciences and Technology, National Engineering Laboratory for Animal Breeding & Key Laboratory of Animal Genetics,Breeding and Reproduction, Ministry of Agriculture, China Agricultural University, Beijing 100193, People's Republic of China;

College of Animal Sciences and Technology, National Engineering Laboratory for Animal Breeding & Key Laboratory of Animal Genetics,Breeding and Reproduction, Ministry of Agriculture, China Agricultural University, Beijing 100193, People's Republic of China;

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Expression of genes involved in progesterone receptor paracrine signaling and their effect on litter size in pigs

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