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Resveratrol compares with melatonin in improving in vitro porcine oocyte maturation under heat stress

机译:白藜芦醇与褪黑素在热应激下改善猪卵母细胞体外成熟的比较

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摘要

Background:Resveratrol,an important phyto-antioxidant commonly found in grapes,mulberry,and other plants,has a variety of functions including anti-aging,anti-cancer and anti-inflammatory activities.In the current study,we investigated the beneficial effects of resveratrol on in vitro porcine oocyte maturation under heat stress (HS).The effect of resveratrol,melatonin and their combination on alleviating HS was compared according to the maturation rate of oocytes and the development competence of embryos after parthenogenetic activation (PA).Results:Supplementation with resveratrol (2.0 μmol/L) not only improved the nuclear maturation but also raised the blastocyst rate of porcine embryos‘ PA from oocytes that underwent HS by increasing their glutathione (GSH) level,reducing reactive oxygen species (ROS) and up-regulating the expression of Sirtuin 1 (SIRT1).It was also found that melatonin (10-7 mol/L) and the combination of resveratrol (2.0 μmol/L) plus melatonin (10-7 mol/L) exhibited more potent effects than resveratrol alone regarding their protective activities on oocyte maturation under HS.Conclusions:This study compared the efficiencies of resveratrol,melatonin and their combination for protecting porcine oocytes from heat stress.The mechanisms are attributed to the fact that each treatment may have different ability to regulate the synthesis of steroid hormones and the expression of mature related genes.
机译:背景:白藜芦醇是葡萄,桑树等植物中常见的重要植物抗氧化剂,具有多种功能,包括抗衰老,抗癌和抗炎活性。在本研究中,我们研究了白藜芦醇的有益作用。白藜芦醇对热应激(HS)下猪卵母细胞成熟的影响补充白藜芦醇(2.0μmol/ L)不仅可以改善核成熟,还可以通过增加其谷胱甘肽(GSH)含量,减少活性氧(ROS)并提高-调节Sirtuin 1(SIRT1)的表达,还发现褪黑激素(10-7 mol / L)和白藜芦醇(2.0μmol/ L)加褪黑素(10-7 mol / L)的组合结论:本研究比较了白藜芦醇,褪黑激素及其组合对保护猪卵母细胞免受热应激的功效。该机理归因于每个处理可能具有调节类固醇激素合成和成熟相关基因表达的能力不同。

著录项

  • 来源
    《畜牧与生物技术杂志(英文版)》 |2017年第1期|89-98|共10页
  • 作者单位

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

    College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China;

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

    National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
  • 正文语种 eng
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