Methodological factors affecting gas and methane production during in vitro rumen fermentation evaluated by meta-analysis approach

Laura Maccarana; Mirko Cattani; Franco Tagliapietra; Stefano Schiavon; Lucia Bailoni; Roberto Mantovani

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Methodological factors affecting gas and methane production during in vitro rumen fermentation evaluated by meta-analysis approach

机译：荟萃分析法评估影响瘤胃体外发酵过程中气体和甲烷产生的方法学因素

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Effects of some methodological factors on in vitro measures of gas production (GP,mL/g DM),CH4 production (mL/g DM) and proportion (％ CH4 on total GP) were investigated by meta-analysis.These factors were considered:pressure in the GP equipment (0 =constant;1 =increasing),incubation time (0 =24;1 =≥ 48 h),time of rumen fluid collection (0 =before feeding;1 =after feeding of donor animals),donor species of rumen fluid (0 =sheep;1 =bovine),presence of N in the buffer solution (0 =presence;1 =absence),and ratio between amount of buffered rumen fluid and feed sample (BRF/FS;0 =≤ 130 mL/g DM;1 =130-140 mL/g DM;2=≥ 140 mL/g DM).The NDF content of feed sample incubated (NDF) was considered as a continuous variable.From an initial database of 105 papers,58 were discarded because one of the above-mentioned factors was not stated.After discarding 17 papers,the final dataset comprised 30 papers (339 observations).A preliminary mixed model analysis was carried out on experimental data considering the study as random factor.Variables adjusted for study effect were analyzed using a backward stepwise analysis including the above-mentioned variables.The analysis showed that the extension of incubation time and reduction of NDF increased GP and CH4 values.Values of GP and CH4 also increased when rumen fluid was collected after feeding compared to before feeding (+26.4 and +9.0 mL/g DM,for GP and CH4),from bovine compared to sheep (+32.8 and +5.2 mL/g DM,for GP and CH4),and when the buffer solution did not contain N (+24.7 and +6.7 mL/g DM for GP and CH4).The increase of BRF/FS ratio enhanced GP and CH4 production (+7.7 and +3.3 mL/g DM per each class of increase,respectively).In vitro techniques for measuring GP and CH4 production are mostly used as screening methods,thus a full standardization of such techniques is not feasible.However,a greater harmonization of analytical procedures (i.e.,a reduction in the number of available protocols) would be useful to facilitate comparison between results of different experiments.

机译：通过荟萃分析，研究了一些方法学因素对体外气体生成量（GP，mL / g DM），CH4生成量（mL / g DM）和比例（占总GP的百分比CH4）的影响。这些因素包括： GP设备的压力（0 =恒定; 1 =增加），孵育时间（0 = 24; 1 =≥48 h），瘤胃液收集时间（0 =喂养前; 1 =喂养供体动物后）瘤胃液种类（0 =绵羊; 1 =牛），缓冲液中N的存在（0 =存在; 1 =缺乏），缓冲的瘤胃液与进料样品的比例（BRF / FS; 0 =≤ 130 mL / g DM; 1 = 130-140 mL / g DM; 2 =≥140 mL / g DM）。孵育的饲料样品（NDF）的NDF含量被视为连续变量。来自105篇论文的初始数据库由于未提及上述因素之一，因此丢弃了58篇论文。丢弃17篇论文后，最终数据集包括30篇论文（339个观察值）。考虑了实验数据，对模型进行了初步的混合模型分析使用包括上述变量在内的向后逐步分析方法对因研究效果而调整的变量进行了分析，分析结果表明孵育时间的延长和NDF的减少会增加GP和CH4的值，GP和CH4的值也是如此与牛相比，饲喂后收集的瘤胃液与饲喂前相比增加（对于GP和CH4，分别为+26.4和+9.0 mL / g DM），对于绵羊，相比于喂养前（+32.8和+5.2 mL / g DM，对于GP和CH4），并且当缓冲液中不含N时（GP和CH4的DM分别为+24.7和+6.7 mL / g DM）。BRF/ FS比的增加提高了GP和CH4的产生（每个DM分别为7.7和+3.3 mL / g DM）分别使用测量GP和CH4产生的体外技术作为筛选方法，因此对这些技术进行完全标准化是不可行的。但是，要实现更大程度的统一分析程序（即减少数量）的可用协议）将有助于进行比较补间不同实验的结果。

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《畜牧与生物技术杂志(英文版)》 |2017年第1期|236-247|共12页
作者
Laura Maccarana; Mirko Cattani; Franco Tagliapietra; Stefano Schiavon; Lucia Bailoni; Roberto Mantovani;
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Department of Comparative Biomedicine and Food Science(BCA),University of Padova, Viale dell'Università 16, 35020 Legnaro(PD), Italy;

Department of Comparative Biomedicine and Food Science(BCA),University of Padova, Viale dell'Università 16, 35020 Legnaro(PD), Italy;

Department of Agronomy, Food, Natural resources, Animals and Environment(DAFNAE), University of Padova, Viale dell'Università 16, 35020 Legnaro(PD), Italy;

Department of Agronomy, Food, Natural resources, Animals and Environment(DAFNAE), University of Padova, Viale dell'Università 16, 35020 Legnaro(PD), Italy;

Department of Comparative Biomedicine and Food Science(BCA),University of Padova, Viale dell'Università 16, 35020 Legnaro(PD), Italy;

Department of Agronomy, Food, Natural resources, Animals and Environment(DAFNAE), University of Padova, Viale dell'Università 16, 35020 Legnaro(PD), Italy;

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Methodological factors affecting gas and methane production during in vitro rumen fermentation evaluated by meta-analysis approach

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