Proteomic identification of cryostress in epididymal spermatozoa

Sung Jae Yoon; Md Saidur Rahman; Woo Sung Kwon; Do Yeal Ryu; Yoo Jin Park; Myung Geol Pang

首页> 外文期刊>畜牧与生物技术杂志(英文版) >Proteomic identification of cryostress in epididymal spermatozoa

【24h】

Proteomic identification of cryostress in epididymal spermatozoa

机译：附睾精子中冷冻应激的蛋白质组学鉴定

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Backgroune:Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods,as the sudden death of an animal or a catastrophic injury.However,the freezing and thawing processes cause stress to spermatozoa,including cold shock,osmotic damage,and ice crystal formation,thereby reducing sperm quality,We assessed the motility (％),motion kinematics,capacitation status,and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining.Moreover,we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis (2-DE) results using two commercial antibodies.Results:Cryopreservation reduced viability (％),motility (％),straight-line velocity (VSL),average path velocity (VAP),amplitude of lateral head displacement (ALH),and capacitated spermatozoa,whereas straightness (STR) and the acrosome reaction increased after cryopreservation (P ＜ 0.05).Nine proteins were differentially expressed (two proteins decreased and seven increased) (＞3 fold,P ＜ 0.05) before and after cryopreservation.The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways,including the ephrinR-actin pathway,the ROS metabolism pathway,actin cytoskeleton assembly,actin cytoskeleton regulation,and the guanylate cyclase pathway.Conclusion:The results of the current study provide information on epididymal sperm proteome dynamics and possible protein markers of cryo-stress during cryopreservation.This information will further the basic understanding of cryopreservation and aid future studies aiming to identify the mechanism of cryostress responses.

机译：背景：对于不能用常规方法收集精液的情况，如动物的突然死亡或灾难性伤害，冷冻保存附睾精子非常重要。但是，冷冻和解冻过程会导致精子受压，包括冷休克，渗透损伤和冰晶形成，从而降低精子质量，我们使用计算机辅助精子分析和Hoechst 33258 /氯四环素荧光染色法评估了精子的活力（％），运动学，获能状态和生存力。蛋白通过蛋白质组学方法与低温应激相关蛋白进行蛋白质印迹，并使用两种市售抗体进行了二维电泳（2-DE）结果验证。冷冻保存降低了活力（％），活力（％），直线速度（VSL），平均路径速度（VAP），头部侧向位移幅度（ALH）和精子获能，而直线度（STR）和顶体r低温保存后的反应增加（P ＜0.05）。低温保存前后差异表达的蛋白质有9种（减少2种，增加7种）（＞ 3倍，P ＜0.05）。包括ephrinR-肌动蛋白途径，ROS代谢途径，肌动蛋白细胞骨架装配，肌动蛋白细胞骨架调控和鸟苷酸环化酶途径。结论：本研究结果提供了附睾精子蛋白质组动力学和低温应激可能的蛋白质标志物的信息。这些信息将进一步加深对冷冻保存的基本了解，并有助于旨在识别冷冻应激反应机理的未来研究。

著录项

来源
《畜牧与生物技术杂志(英文版)》 |2017年第2期|478-489|共12页
作者
Sung Jae Yoon; Md Saidur Rahman; Woo Sung Kwon; Do Yeal Ryu; Yoo Jin Park; Myung Geol Pang;
展开▼
作者单位

Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-Do 456-756, Republic of Korea;

Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-Do 456-756, Republic of Korea;

Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-Do 456-756, Republic of Korea;

Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-Do 456-756, Republic of Korea;

Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-Do 456-756, Republic of Korea;

Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-Do 456-756, Republic of Korea;

展开▼
收录信息中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 eng
中图分类
关键词

Proteomic identification of cryostress in epididymal spermatozoa

摘要

著录项

相关主题

期刊订阅