Breed and adaptive response modulate bovine peripheral blood cells' transcriptome

Nataliya Po(s)(c)i(c); Tommaso Montanari; Mariasilvia DAndrea; Danilo Licastro; Fabio Pilla; Paolo Ajmone-Marsan; Andrea Minuti; Sandy Sgorlon

首页> 外文期刊>畜牧与生物技术杂志(英文版) >Breed and adaptive response modulate bovine peripheral blood cells' transcriptome

【24h】

Breed and adaptive response modulate bovine peripheral blood cells' transcriptome

机译：品种和适应性反应调节牛外周血细胞的转录组

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Background:Adaptive response includes a variety of physiological modifications to face changes in external or internal conditions and adapt to a new situation.The acute phase proteins (APPs) are reactants synthesized against environmental stimuli like stress,infection,inflammation.Methods:To delineate the differences in molecular constituents of adaptive response to the environment we performed the whole-blood transcriptome analysis in Italian Holstein (IH) and Italian Simmental (IS) breeds.For this,663 IH and IS cows from six commercial farms were clustered according to the blood level of APPs.Ten extreme individuals (five APP+ and APP-variants) from each farm were selected for the RNA-seq using the Illumina sequencing technology.Differentially expressed (DE) genes were analyzed using dynamic impact approach (DIA) and DAVID annotation clustering.Milk production data were statistically elaborated to assess the association of APP+ and APP-gene expression patterns with variations in milk parameters.Results:The overall de novo assembly of cDNA sequence data generated 13,665 genes expressed in bovine blood cells.Comparative genomic analysis revealed 1,152 DE genes in the comparison of all APP+ vs.all APP-variants;531 and 217 DE genes specific for IH and IS comparison respectively.In all comparisons overexpressed genes were more represented than underexpressed ones.DAVID analysis revealed 369 DE genes across breeds,173 and 73 DE genes in IH and IS comparison respectively.Among the most impacted pathways for both breeds were vitamin B6 metabolism,folate biosynthesis,nitrogen metabolism and linoleic acid metabolism.Conclusions:Both DIA and DAVID approaches produced a high number of significantly impacted genes and pathways with a narrow connection to adaptive response in cows with high level of blood APPs.A similar variation in gene expression and impacted pathways between APP+ and APP-variants was found between two studied breeds.Such similarity was also confirmed by annotation clustering of the DE genes.However,IH breed showed higher and more differentiated impacts compared to IS breed and such particular features in the IH adaptive response could be explained by its higher metabolic activity.Variations of milk production data were significantly associated with APP+ and APP-gene expression patterns.

机译：背景：适应性反应包括多种生理变化，以适应外部或内部条件的变化并适应新情况。急性期蛋白（APP）是针对压力，感染，炎症等环境刺激而合成的反应物。方法：我们对意大利荷斯坦（IH）和意大利西门塔尔（IS）品种进行了全血转录组分析。为此，来自六个商业农场的663头IH和IS母牛根据血液进行了聚类通过使用Illumina测序技术从每个农场中选择10个极端个体（五个APP +和APP变体）进行RNA测序，使用动态影响法（DIA）和DAVID注释聚类分析差异表达（DE）基因对牛奶生产数据进行统计学处理，以评估APP +和APP基因表达模式与牛奶变化之间的关联结果：从头到尾的cDNA序列数据的整体组装产生了在牛血细胞中表达的13,665个基因。通过比较基因组分析，在所有APP +与所有APP变体的比较中，共有1,152个DE基因; IH的531和217 DE基因在所有比较中，过表达基因的表达均高于过表达基因的表达.DAVID分析揭示了跨品种的369个DE基因，在IH和IS比较中分别为173个和73个DE基因。两个品种中受影响最大的途径是维生素B6代谢结论：DIA和DAVID两种方法都能产生大量受到显着影响的基因，并且在血液水平较高的APP的奶牛中与适应性反应的联系狭窄。基因表达的相似变化并且在两个研究品种之间发现了APP +和APP变异之间的影响途径。然而，与IS品种相比，IH品种表现出越来越高的差异化影响，并且IH适应性反应中的此类特殊特征可以用其较高的代谢活性来解释。牛奶产量数据的变化与APP +和APP基因表达模式。

著录项

来源
《畜牧与生物技术杂志(英文版)》 |2017年第2期|335-350|共16页
作者
Nataliya Po(s)(c)i(c); Tommaso Montanari; Mariasilvia DAndrea; Danilo Licastro; Fabio Pilla; Paolo Ajmone-Marsan; Andrea Minuti; Sandy Sgorlon;
展开▼
作者单位

Department of Agriculture, Food, Environment and Animal Science(DI4A), University of Udine, via delle Scienze 206, 33100 Udine, Italy;

Department of Agriculture, Food, Environment and Animal Science(DI4A), University of Udine, via delle Scienze 206, 33100 Udine, Italy;

Department of Agricultural, Environmental and Food Sciences, University of Molise, via F.De Sanctis snc, 86100 Campobasso, Italy;

CBM S.c.r.l, SS 14-km 163.5 AREA Science Park, 34149 Basovizza, TS, Italy;

Department of Agricultural, Environmental and Food Sciences, University of Molise, via F.De Sanctis snc, 86100 Campobasso, Italy;

Institute of Zootechnics, Catholic University of the Sacred Heart, via Emilia Parmense 84, 29133 Piacenza, Italy;

Institute of Zootechnics, Catholic University of the Sacred Heart, via Emilia Parmense 84, 29133 Piacenza, Italy;

Department of Agriculture, Food, Environment and Animal Science(DI4A), University of Udine, via delle Scienze 206, 33100 Udine, Italy;

展开▼
收录信息中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 eng
中图分类
关键词

Breed and adaptive response modulate bovine peripheral blood cells' transcriptome

摘要

著录项

相关主题

期刊订阅