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首页> 外文期刊>畜牧与生物技术杂志(英文版) >Regulatory elements and transcriptional control of chicken vasa homologue (CVH) promoter in chicken primordial germ cells
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Regulatory elements and transcriptional control of chicken vasa homologue (CVH) promoter in chicken primordial germ cells

机译：鸡原始生殖细胞中鸡Vasa同源物（CVH）启动子的调控元件和转录控制

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Background:Primordial germ cells (PGCs),the precursors of functional gametes,have distinct characteristics and exhibit several unique molecular mechanisms to maintain pluripotency and germness in comparison to somatic cells.They express germ cell-specific RNA binding proteins (RBPs) by modulating tissue-specific cis-and trans-regulatory elements.Studies on gene structures of chicken vasa homologue (CVH),a chicken RNA binding protein,involved in temporal and spatial regulation are thus important not only for understanding the molecular mechanisms that regulate germ cell fate,but also for practical applications of primordial germ cells.However,very limited studies are available on regulatory elements that control germ cell-specific expression in chicken.Therefore,we investigated the intricate regulatory mechanism(s) that governs transcriptional control of CVH.Results:We constructed green fluorescence protein (GFP) or luciferase reporter vectors containing the various 5'flanking regions of CVH gene.From the 5'deletion and fragmented assays in chicken PGCs,we have identified a CVH promoter that locates at-316 to +275 base pair fragment with the highest luciferase activity.Additionally,we confirmed for the first time that the 5'untranslated region (UTR) containing intron 1 is required for promoter activity of the CVH gene in chicken PGCs.Furthermore,using a transcription factor binding prediction,transcriptome analysis and siRNA-mediated knockdown,we have identified that a set of transcription factors play a role in the PGC-specific CVH gene expression.Conclusions:These results demonstrate that cis-elements and transcription factors localizing in the 5'flanking region including the 5'UTR and an intron are important for transcriptional regulation of the CVH gene in chicken PGCs.Finally,this information will contribute to research studies in areas of reproductive biology,constructing of germ cell-specific synthetic promoter for tracing primordial germ cells as well as understanding the transcriptional regulation for maintaining germness in PGCs.
机译：背景：功能性配子的前体生殖细胞（PGCs）具有独特的特征，并且与体细胞相比具有多种独特的分子机制来维持多能性和生殖力。它们通过调节组织来表达生殖细胞特异的RNA结合蛋白（RBPs）。特异性的顺式和反式调控元件。涉及鸡RNA结合蛋白的鸡瓦萨同源基因（CVH）的基因结构研究不仅涉及了解调控生殖细胞命运的分子机制，而且还涉及时间和空间调控，但是，对于控制鸡生殖细胞特异性表达的调控元件的研究还非常有限。因此，我们研究了控制CVH转录调控的复杂调控机制。我们构建了绿色荧光蛋白（GFP）或荧光素酶报告载体，其中包含各种5'侧翼区域f CVH基因。通过在鸡PGC中进行5'缺失和片段化分析，我们鉴定出了一个CVH启动子，该启动子位于荧光素酶活性最高的316至+275个碱基对片段。此外，我们首次确认了5个鸡PGC中CVH基因的启动子活性需要含有内含子1的非翻译区（UTR）。此外，使用转录因子结合预测，转录组分析和siRNA介导的敲低，我们已经确定了一组转录因子在小鼠中起着重要作用。结论：这些结果表明，顺式元件和转录因子位于5'侧翼区域（包括5'UTR和一个内含子）对于鸡PGCs中CVH基因的转录调控非常重要。最后，这些信息将有助于生殖生物学领域的研究，构建生殖细胞特异性合成启动子以追踪原始生殖细胞以及在维持PGCs萌发的转录调控方面颇有建树。

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  • 来源
    《畜牧与生物技术杂志(英文版)》 |2017年第2期|490-500|共11页
  • 作者

    So Dam Jin; Bo Ram Lee; Young Sun Hwang; Hong Jo Lee; Jong Seop Rim; Jae Yong Han;

  • 作者单位

    Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, South Korea;

    Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, South Korea;

    Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, South Korea;

    Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, South Korea;

    Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, South Korea;

    Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, South Korea;

    Institute for Biomedical Sciences, Shinshu University, Minamiminowa, Nagano 399-4598, Japan;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
  • 正文语种 eng
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