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Novel SNPs in IL-17F and IL-17A genes associated with somatic cell count in Chinese Holstein and Inner-Mongolia Sanhe cattle

机译:与中国荷斯坦牛和内蒙古三河牛体细胞计数相关的IL-17F和IL-17A基因中的新型SNP

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摘要

Background:Bovine mastitis is the most common and costly disease of lactating cattle worldwide.Apart from milk somatic cell count (SCC) and somatic cell score (SCS),serum cytokines such as interleukinq 7 (IL-17) and interleukin-4 (IL-4) may also be potential indicators for bovine mastitis.The present study was designed to investigate the effects of single nucleotide polymorphisms (SNPs) in bovine IL-17F and IL-17A genes on SCC,SCS and serum cytokines in Chinese Holstein and Inner-Mongolia Sanhe cattle,and to compare the mRNA expression variations of the cows with different genotypes.Results:A total of 464 lactating cows (337 Holstein and 1 27 Inner-Mongolia Sanhe cattle) were screened for SNPs identification and the data were analyzed using fixed effects of herd,parity,season and year of calving by general linear model procedure.The results revealed that SNP g.24392436C >T in IL-17F and SNP g.24345410A > G in IL-17A showed significant effects on SCC and IL-4 in Holstein (n =337) and on IL-17 and IL-4 in Sanhe cattle (n =127).The homozygous GG genotype of SNP g.24345410A > G had significantly higher mRNA expression compared with the heterozygous AG genotype.Conclusions:The results indicate that IL-17F and IL-17A could be powerful candidate genes of mastitis resistance and the significant SNPs might be useful genetic markers against mastitis in both dairy and dual purpose cattle.
机译:背景:牛乳腺炎是全世界泌乳牛最常见且代价最高的疾病。除了乳体细胞计数(SCC)和体细胞评分(SCS)之外,血清细胞因子如白介素7(IL-17)和白介素4(IL) -4)可能也是牛乳腺炎的潜在指标。本研究旨在研究牛IL-17F和IL-17A基因中的单核苷酸多态性(SNP)对中国荷斯坦和内蒙古地区SCC,SCS和血清细胞因子的影响结果:共筛选了464头泌乳牛(337头荷斯坦奶牛和1头27只内蒙古三河奶牛)进行SNPs鉴定,并利用数据进行了分析。结果表明,IL-17F的SNP g.24392436C> T和IL-17A的SNP g.24345410A> G对SCC和IL具有显着影响-4在Holstein(n = 337)及以上三河牛IL-17和IL-4(n = 127)。SNPg.24345410A> G的纯合GG基因型与杂合AG基因型相比,mRNA表达明显升高。结论:IL-17F和IL -17A可能是抵抗乳腺炎的强大候选基因,而重要的SNP可能是奶牛和两用牛抵抗乳腺炎的有用遗传标记。

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  • 来源
    《畜牧与生物技术杂志(英文版)》 |2017年第2期|361-369|共9页
  • 作者单位

    Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China;

    College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan;

    Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China;

    Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China;

    Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China;

    Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China;

    Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China;

    Xieerltala Breeding Farm, Hailaer 021012, Inner Mongolia, China;

    Agricultural and Animal Husbandry Administration Bureau, Hailaer 021000, Inner Mongolia, China;

    Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
  • 正文语种 eng
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