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首页> 外文期刊>畜牧与生物技术杂志(英文版) >Effects of Bacillus subtilis CSL2 on the composition and functional diversity of the faecal microbiota of broiler chickens challenged with Salmonella Gallinarum
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Effects of Bacillus subtilis CSL2 on the composition and functional diversity of the faecal microbiota of broiler chickens challenged with Salmonella Gallinarum

机译：枯草芽孢杆菌CSL2对鸡沙门氏菌挑战的肉鸡粪便菌群组成和功能多样性的影响

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摘要

Background:The chicken gastrointestinal tract contains a diverse microbiota whose composition and structure play important roles in gut functionality.In this study,microbial shifts resulting from feed supplementation with Bacillus subtilis CSL2 were evaluated in broilers challenged and unchallenged with Salmonella Gallinarum.To analyse bacterial community composition and functionality,454 GS-FLX pyrosequencing of 16S rRNA gene amplicons was performed.Results:The Quantitative Insights into Microbial Ecology (QIIME) pipeline was used to analyse changes in the faecal microbiota over a 24-h period.A total of 718,204 sequences from broiler chickens were recorded and analysed.At the phylum level,Firmicutes,Bacteroidetes,and Proteobacteria were the predominant bacterial taxa.In Salmonella-infected chickens (SC),Bacteroidetes were more highly abundant compared to control (NC) and Bacillus-treated (BT) chickens.At the genus level,in the NC and BT groups,Lactobacillus was present at high abundance,and the abundance of Turicibacter,unclassified Enterobacteriaceae,and Bacteroides increased in SC broilers.Furthermore,taxon-independent analysis showed that the SC and BT groups were compositionally distinct at the end of the 24-h period.Further analysis of functional properties showed that B.subtilis CSL2 administration increased gut-associated energy supply mechanisms (i.e.carbohydrate transport and metabolism) to maintain a stable microbiota and protect gut integrity.Conclusions:This study demonstrated that S.Gallinarum infection and B.subtilis CSL2 supplementation in the diet of broiler chickens influenced the diversity,composition,and functional diversity of the faecal microbiota.Moreover,the findings offer significant insights to understand potential mechanisms of Salmonella infection and the mode of action of probiotics in broiler chickens.
机译：背景：鸡胃肠道包含多种微生物群，其组成和结构在肠道功能中起着重要作用。组成和功能，对16S rRNA基因扩增子进行454 GS-FLX焦磷酸测序。结果：微生物生态学定量研究（QIIME）用于分析24小时内粪便微生物群的变化，共718,204个序列记录并分析了来自肉鸡的细菌。在菌门水平上，Firmicutes，Bacteroidetes和Proteobacteria是主要的细菌类群。在沙门氏菌感染的鸡（SC）中，与对照（NC）和经芽孢杆菌处理（ BT）鸡。在属水平上，在NC和BT组中，乳酸杆菌的含量很高ce肉鸡中ce的含量增加，并且Tur细菌，未分类的肠杆菌科细菌和拟杆菌的数量增加。此外，不依赖分类群的分析表明，SC和BT组在24小时结束时组成上是不同的。对功能特性的进一步分析表明枯草芽孢杆菌CSL2的施用增加了肠道相关的能量供应机制（即碳水化合物的运输和代谢），以维持稳定的微生物群并保护肠道完整性。结论：本研究表明，在饮食中添加了S.Gallinarum感染和补充了枯草芽孢杆菌CSL2。肉鸡影响了粪便微生物群的多样性，组成和功能多样性。此外，该发现为了解沙门氏菌感染的潜在机制和益生菌在鸡中的作用方式提供了重要的见识。

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  • 来源
    《畜牧与生物技术杂志(英文版)》 |2017年第2期|450-458|共9页
  • 作者

    Ju Kyoung Oh; Edward Alain B.Pajarillo; Jong Pyo Chae; In Ho Kim; Dang Soo Yang; Dae-Kyung Kang;

  • 作者单位

    Department of Animal Resources Science, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea;

    Department of Animal Resources Science, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea;

    Department of Animal Resources Science, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea;

    Department of Animal Resources Science, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea;

    Abson BioChem, Inc, 10-1 Yangjimaeul-gil, Sangrok-gu, Ansan 15524, Republic of Korea;

    Department of Animal Resources Science, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea;

  • 收录信息 中国科学引文数据库(CSCD);
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  • 正文语种 eng
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