Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection

Jin Tang; Wenzhuo Hao; Ruiping She; Xun Suo; Qiaoxing Wu; Xinming Tang; Ruihan Shi; Jingxia Suo; Guangping Huang; Junqing An; Jingyuan Wang; Jinling Yang

首页> 外文期刊>畜牧与生物技术杂志(英文版) >Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection

【24h】

Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection

机译：体内兔结扎肠环模型感染HCMV的开发

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Background:Human Cytomegalovirus (HCMV) infections can be found throughout the body,especially in epithelial tissue.Animal model was established by inoculation of HCMV (strain AD-169) or coinoculation with Hepatitis E virus (HEV) into the ligated sacculus rotundus and vermiform appendix in living rabbits.The specimens were collected from animals sacrificed 1 and a half hours after infection.Results:The virus was found to be capable of reproducing in these specimens through RT-PCR and Western-blot.Severe inflammation damage was found in HCMV-infected tissue.The viral protein could be detected in high amounts in the mucosal epithelium and lamina propria by immunohistochemistry and immunofluorescense.Moreover,there are strong positive signals in lymphocytes,macrophages,and lymphoid follicles.Quantitative statistics indicate that lymphocytes among epithlium cells increased significantly in viral infection groups.Conclusions:The results showed that HCMV or HEV + HCMV can efficiently infect in rabbits by vivo ligated intestine loop inoculation.The present study successfully developed an infective model in vivo rabbit ligated intestinal Loop for HCMV pathogenesis study.This rabbit model can be helpful for understanding modulation of the gut immune system with HCMV infection.

机译：背景：人类巨细胞病毒（HCMV）感染遍布全身，特别是在上皮组织中。通过接种HCMV（AD-169株）或与戊型肝炎病毒（HEV）共接种到结扎的圆形结节和ver状虫中，建立了动物模型。结果：通过RT-PCR和Western-blot发现病毒能够在这些标本中繁殖，在HCMV中发现了严重的炎症损害通过免疫组织化学和免疫荧光检测，可以在粘膜上皮和固有层中大量检测到病毒蛋白。此外，淋巴细胞，巨噬细胞和淋巴滤泡中都有强阳性信号。定量统计表明上皮细胞中的淋巴细胞增多结论：结果表明，HCMV或HEV + HCMV可以有效感染本研究成功建立了家兔结扎肠Loop的感染模型，用于HCMV发病机理研究。该兔模型有助于理解HCMV感染对肠道免疫系统的调节作用。

著录项

来源
《畜牧与生物技术杂志(英文版)》 |2017年第3期|738-749|共12页
作者
Jin Tang; Wenzhuo Hao; Ruiping She; Xun Suo; Qiaoxing Wu; Xinming Tang; Ruihan Shi; Jingxia Suo; Guangping Huang; Junqing An; Jingyuan Wang; Jinling Yang;
展开▼
作者单位

National animal protozoa laboratory,College of VeterinaryMedicine,China Agricultural University,Beijing 100193,China;

Laboratory of Animal Pathology & Public Health,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;

Laboratory of Animal Pathology & Public Health,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;

National animal protozoa laboratory,College of VeterinaryMedicine,China Agricultural University,Beijing 100193,China;

Laboratory of Animal Pathology & Public Health,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;

National animal protozoa laboratory,College of VeterinaryMedicine,China Agricultural University,Beijing 100193,China;

Laboratory of Animal Pathology & Public Health,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;

National animal protozoa laboratory,College of VeterinaryMedicine,China Agricultural University,Beijing 100193,China;

National animal protozoa laboratory,College of VeterinaryMedicine,China Agricultural University,Beijing 100193,China;

Laboratory of Animal Pathology & Public Health,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;

Laboratory of Animal Pathology & Public Health,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;

Laboratory of Animal Pathology & Public Health,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;

展开▼
收录信息中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 eng
中图分类
关键词

Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection

摘要

著录项

相关主题

期刊订阅