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New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models

机译：评估精子DNA片段动力学的新方法：微调数学模型

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Background:Sperm DNA fragmentation (sDF) has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample.Colloid centrifugation could be a suitable technique to select those donkey sperm more resistant to DNA fragmentation after thawing.Previous studies have shown that to elucidate the latent damage of the DNA molecule,sDF should be assessed dynamically,where the rate of fragmentation between treatments indicates how resistant the DNA is to iatrogenic damage.The rate of fragmentation is calculated using the slope of a linear regression equation.However,it has not been studied if sDF dynamics fit this model.The objectives of this study were to evaluate the effect of different after-thawing centrifugation protocols on sperm DNA fragmentation and elucidate the most accurate mathematical model (linear regression,exponential or polynomial) for DNA fragmentation over time in frozen-thawed donkey semen.Results:After submitting post-thaw semen samples to no centrifugation (UDC),sperm washing (SW) or single layer centrifugation (SLC) protocols,sDF values after 6 h of incubation were significantly lower in SLC samples than in SW or UDC.Coefficient of determination (R2) values were significantly higher for a second order polynomial model than for linear or exponential.The highest values for acceleration of fragmentation (aSDF) were obtained for SW,followed by SLC and UDC.Conclusion:SLC after thawing seems to preserve longer DNA longevity in comparison to UDC and SW.Moreover,the fine-tuning of models has shown that sDF dynamics in frozen-thawed donkey semen fit a second order polynomial model,which implies that fragmentation rate is not constant and fragmentation acceleration must be taken into account to elucidate hidden damage in the DNA molecule.
机译：背景：精子DNA片段化（sDF）已被证明是一个重要参数，可以在体外预测精液样本的潜在生育力。胶体离心法可能是一种合适的技术，用于选择解冻后对DNA片段更耐性的那些驴子精子。先前的研究表明，为了阐明DNA分子的潜在损伤，应动态评估sDF，其中两次治疗之间的裂解率表明DNA对医源性损伤的抵抗力。裂解率使用线性斜率计算回归方程。但是，尚未研究sDF动力学是否适合该模型。本研究的目的是评估不同解冻后离心方案对精子DNA片段化的影响，并阐明最准确的数学模型（线性回归，指数回归或多项式）随着时间的流逝在冻融的驴精液中发生DNA片段化。结果：提交解冻后的精液后在无离心（UDC），精子洗涤（SW）或单层离心（SLC）方案的n个样品中，孵育6小时后，sLC样品中的sDF值显着低于SW或UDC。测定系数（R2）值为二阶多项式模型明显高于线性或指数模型。SW的碎裂加速（aSDF）最高，随后是SLC和UDC。结论：与UDC相比，融化后的SLC似乎保留了更长的DNA寿命。此外，模型的微调表明，冻融驴精液中的sDF动态符合二阶多项式模型，这意味着碎裂速率不是恒定的，必须考虑碎裂加速度，以阐明石棉中的潜在破坏。 DNA分子。

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  • 来源
    《畜牧与生物技术杂志(英文版)》 |2017年第3期|592-600|共9页
  • 作者

    Isabel Ortiz; Jesús Dorado; Jane Morrell; Jaime Gosálvez; Francisco Crespo; Juan M.Jiménez; Manuel Hidalgo;

  • 作者单位

    Veterinary Reproduction Group,Department of Animal Medicine and Surgery,Faculty of Veterinary Medicine,University of Cordoba,14071 Cordoba,Spain;

    Veterinary Reproduction Group,Department of Animal Medicine and Surgery,Faculty of Veterinary Medicine,University of Cordoba,14071 Cordoba,Spain;

    Department of Clinical Sciences,Division of Reproduction,Swedish University of Agricultural Sciences,Box 7054 SE-75007 Uppsala,Sweden;

    Department of Biology,Universidad Autónoma de Madrid,28049 Madrid,Spain;

    Department of Reproduction,Centro Militar de Cría Caballar(FESCCR Ministry of Defense),05005(A)vila,Spain;

    Department of Chemistry and Physics of Materials,University of Salzburg,Hellbrunner Straβe 34/Ⅲ,A-5020 Salzburg,Austria;

    Veterinary Reproduction Group,Department of Animal Medicine and Surgery,Faculty of Veterinary Medicine,University of Cordoba,14071 Cordoba,Spain;

  • 收录信息 中国科学引文数据库(CSCD);
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