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首页> 外文期刊>畜牧与生物技术杂志(英文版) >Comparison of rumen bacteria distribution in original rumen digesta, rumen liquid and solid fractions in lactating Holstein cows
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Comparison of rumen bacteria distribution in original rumen digesta, rumen liquid and solid fractions in lactating Holstein cows

机译：泌乳荷斯坦奶牛原始瘤胃消化物中瘤胃细菌分布，瘤胃液体和固体部分的比较

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摘要

Background:Original rumen digesta,rumen liquid and solid fractions have been frequently used to assess the rumen bacterial community.However,bacterial profiles in rumen original digesta,liquid and solid fractions vary from each other and need to be better established.Methods:To compare bacterial profiles in each fraction,samples of rumen digesta from six cows fed either a high fiber diet (HFD) or a high energy diet (HED) were collected via rumen fistulas.Rumen digesta was then squeezed through four layers of cheesecloth to separate liquid and solid fractions.The bacterial profiles of rumen original digesta,liquid and solid fractions were analyzed with High-throughput sequencing technique.Results:Rumen bacterial diversity was mainly affected by diet and individual cow (P ＞ 0.05) rather than rumen fraction.Bias distributed bacteria were observed in solid and liquid fractions of rumen content using Venn diagram and LEfSe analysis.Fifteen out of 16 detected biomarkers (using LEfSe analysis) were found in liquid fraction,and these 15 biomarkers contributed the most to the bacterial differences among rumen content fractions.Conclusions:Similar results were found when using samples of original rumen digesta,rumen liquid or solid fractions to assess diversity of rumen bacteria;however,more attention should be draw onto bias distributed bacteria in different ruminal fractions,especially when liquid fraction has been used as a representative sample for rumen bacterial study.
机译：背景：瘤胃的原始消化物，瘤胃的液体和固体部分经常被用来评估瘤胃细菌群落。但是，瘤胃原始消化物中的细菌谱，液体和固体部分彼此不同，需要更好地建立。方法：比较通过瘤胃瘘收集六头高纤维日粮或高能量日粮的奶牛的瘤胃消化物，然后通过四层粗棉布挤压瘤胃消化物以分离液体和采用高通量测序技术分析瘤胃原始消化物，液体和固体部分的细菌谱。使用Venn图和LEfSe分析观察到的瘤胃固形和液相分数.16种检测到的生物标记物中有15种（使用LEfSe分析）结论）：使用原始瘤胃消化物，瘤胃液体或固体组分样品评估瘤胃细菌多样性时，发现了相似的结果，这15种生物标志物对瘤胃含量组分之间细菌差异的贡献最大，应特别注意不同瘤胃部分中的偏性分布细菌，尤其是当液体部分已用作瘤胃细菌研究的代表性样品时。

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  • 来源
    《畜牧与生物技术杂志(英文版)》 |2017年第3期|636-642|共7页
  • 作者

    Shoukun Ji; Hongtao Zhang; Hui Yan; Arash Azarfar; Haitao Shi; Gibson Alugongo; Shengli Li; Zhijun Cao; Yajing Wang;

  • 作者单位

    State Key Laboratory of Animal Nutrition,Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control,China Agricultural University,Beijing 100193,China;

    State Key Laboratory of Animal Nutrition,Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control,China Agricultural University,Beijing 100193,China;

    State Key Laboratory of Animal Nutrition,Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control,China Agricultural University,Beijing 100193,China;

    Faculty of Agriculture,Lorestan University,PO Box 465,Khorramabad,Iran;

    State Key Laboratory of Animal Nutrition,Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control,China Agricultural University,Beijing 100193,China;

    State Key Laboratory of Animal Nutrition,Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control,China Agricultural University,Beijing 100193,China;

    State Key Laboratory of Animal Nutrition,Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control,China Agricultural University,Beijing 100193,China;

    State Key Laboratory of Animal Nutrition,Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control,China Agricultural University,Beijing 100193,China;

    State Key Laboratory of Animal Nutrition,Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control,China Agricultural University,Beijing 100193,China;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
  • 正文语种 eng
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