Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low-and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

Chanwit Kaewtapee; Pia Rosenfelder-Kuon; Katharina Burbach; Georgina Tomforde; Thomas Hartinger; Amélia Camarinha-Silva; Sonja Heinritz; Jana Seifert; Markus Wiltafsky; Rainer Mosenthin

首页> 外文期刊>畜牧与生物技术杂志(英文版) >Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low-and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

【24h】

Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low-and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

机译：低蛋白和高蛋白日粮中添加枯草芽孢杆菌和地衣芽孢杆菌对生长猪回肠粗蛋白，氨基酸消化率和肠道菌群组成的影响

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Background:Bacillus spp.seem to be an alternative to antimicrobial growth promoters for improving animals' health and performance.However,there is little information on the effect of Bacillus spp.in combination with different dietary crude protein (CP) levels on the ileal digestibility and microbiota composition.Therefore,the objective of this study was to determine the effect of Bacillus spp.supplementation to low-(LP) and high-protein diets (HP) on ileal CP and amino acid (AA) digestibility and intestinal microbiota composition.Methods:Eight ileally cannulated pigs with an initial body weight of 28.5 kg were randomly allocated to a rowcolumn design with 8 pigs and 3 periods of 16 d each.The assay diets were based on wheat-barley-soybean meal with two protein levels:LP (14％ CP,as-fed) and HP diet (18％ CP,as-fed).The LP and HP diets were supplemented with or without Bacillus spp.at a level of 0.04％ (as-fed).The apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of CP and AA was determined.Bacterial community composition from ileal digesta was analyzed by Illumina amplicon sequencing and quantitative real-time PCR.Data were analyzed as a 2 × 2 factorial design using the GLIMMIX procedures of SAS.Results:The supplementation with Bacillus spp.did not affect both AID and SID of CP and AA in growing pigs.Moreover,there was no difference in AID of CP and AA between HP and LP diets,but SID of cystine,glutamic acid,glycine,and proline was lower (P ＜ 0.05) in pigs fed the HP diets.The HP diets increased abundance of Bifidobacterium spp.and Lactobacillus spp.,(P ＜ 0.05) and by amplicon sequencing the latter was identified as predominant genus in microbiota from HP with Bacillus spp.,whereas dietary supplementation of Bacillus spp.increased (P ＜ 0.05) abundance of Roseburia spp..Conclusions:The HP diet increased abundance of Lactobacillus spp.and Bifidobacterium spp..The supplementation of Bacillus spp.resulted in a higher abundance of healthy gut associated bacteria without affecting ileal CP and AA digestibility,whereas LP diet may reduce the flow of undigested protein to the large intestine of pigs.

机译：背景：芽孢杆菌似乎是抗菌生长促进剂的替代品，可改善动物的健康和生长性能。然而，关于芽孢杆菌与不同日粮粗蛋白（CP）结合对回肠消化率影响的信息很少。因此，本研究的目的是确定补充低（LP）和高蛋白饮食（HP）的芽孢杆菌属种对回肠CP和氨基酸（AA）消化率和肠道微生物群组成的影响。方法：将8头初始体重为28.5 kg的回肠插管猪随机分配至行栏设计，每头猪8头，每三个月16 d。试验日粮以小麦-大麦-豆粕为基础，具有两种蛋白质水平：LP （饲喂量为14％CP）和HP饲料（饲喂量为18％CP）。LP和HP饲料中添加或不添加芽孢杆菌（Bacillus spp。）的水平为0.04％（饲喂）。消化率（AID）和标准化回肠消化率（SI D）确定CP和AA，通过Illumina扩增子测序和实时定量PCR分析回肠消化道的细菌群落组成，并使用SAS的GLIMMIX程序以2×2因子设计分析数据。芽孢杆菌不影响生长猪的CP和AA的AID和SID。此外，HP和LP日粮的CP和AA的AID没有差异，但是胱氨酸，谷氨酸，甘氨酸和脯氨酸的SID较低。饲喂高脂日粮的猪中（P ＜0.05）。高脂日粮增加了双歧杆菌和乳杆菌的丰度（P ＜0.05），并通过扩增子测序确定了后者是芽孢杆菌属HP菌群中的主要属。结论：HP饮食增加了乳酸杆菌和双歧杆菌的丰富度。结论：HP饮食增加了乳酸杆菌和双歧杆菌的丰富度。如细菌不影响回肠的CP和AA消化率，而LP日粮可以减少未消化的蛋白质流向猪大肠的流量。

著录项

来源
《畜牧与生物技术杂志(英文版)》 |2017年第3期|686-700|共15页
作者
Chanwit Kaewtapee; Pia Rosenfelder-Kuon; Katharina Burbach; Georgina Tomforde; Thomas Hartinger; Amélia Camarinha-Silva; Sonja Heinritz; Jana Seifert; Markus Wiltafsky; Rainer Mosenthin;
展开▼
作者单位

University of Hohenheim,Institute of Animal Science,Emil-Wolff-Strasse 10,70599 Stuttgart,Germany;

Department of Animal Science,Faculty of Agriculture,Kasetsart University,50 Ngam Wong Wan Rd,Chatuchak,Bangkok 10900,Thailand;

University of Hohenheim,Institute of Animal Science,Emil-Wolff-Strasse 10,70599 Stuttgart,Germany;

University of Hohenheim,Institute of Animal Science,Emil-Wolff-Strasse 10,70599 Stuttgart,Germany;

University of Hohenheim,Institute of Animal Science,Emil-Wolff-Strasse 10,70599 Stuttgart,Germany;

University of Hohenheim,Institute of Animal Science,Emil-Wolff-Strasse 10,70599 Stuttgart,Germany;

University of Bonn,Institute of Animal Science,Endenicher Allee 15,53115 Bonn,Germany;

University of Hohenheim,Institute of Animal Science,Emil-Wolff-Strasse 10,70599 Stuttgart,Germany;

University of Hohenheim,Institute of Animal Science,Emil-Wolff-Strasse 10,70599 Stuttgart,Germany;

University of Hohenheim,Institute of Animal Science,Emil-Wolff-Strasse 10,70599 Stuttgart,Germany;

Evonik Nutrition & Care GmbH,Rodenbacher Chaussee 4,63457 Hanau-Wolfgang,Germany;

University of Hohenheim,Institute of Animal Science,Emil-Wolff-Strasse 10,70599 Stuttgart,Germany;

展开▼
收录信息中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 eng
中图分类
关键词

Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low-and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

摘要

著录项

相关主题

期刊订阅