Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs

Daniel S.Ramos Angrimani; Marcilio Nichi; Joāo Diego A.Losano; Cristina F.Lucio; Gisele A.Lima Veiga; Márcia V.M.Junqueira Franco; Camila I.Vannucchi

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Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs

机译：犬精子成熟过程中附睾液和精子中的脂肪酸含量

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Background:During sperm maturation,there is a reorganization of fatty acids from plasmatic membrane of the spermatozoa,which allows higher membrane integrity and acquisition of sperm motility.However,the fatty acid profile during sperm maturation remains unclear in dogs.Thus,the aim of this study was to identify the fatty acids from the epididymal spermatozoa and plasma during the sperm maturation,and observed changes in the motility and plasmatic membrane parameters.Twenty one adult dogs were used,subsequently to bilateral orchiectomy and epididymal storage,sperm samples were collected from the different segments of the epididymis.Samples were evaluated for conventional microscopy,computer-assisted motility analysis,sperm plasma membrane permeability and the fatty acid analysis (lipids were extracted,transmethylated and analyzed by chromatography).Results:Caput and corpus sperm showed lower values for the motility variables evaluated and plasmatic membrane integrity,indicating different levels of the fatty acids organization.Saturated,monounsaturated and polyunsaturated fatty acids were in higher concentrations in the spermatozoa from epididymis cauda.Highlighting the presence of caprylic,stearic and docosahexaenoic acids.Conclusions:These findings demonstrate the influence of the fatty acid profile during sperm maturation,assigning physical and chemical changes in sperm cells,essential for fertilization.

机译：背景：在精子成熟过程中，精子质膜中的脂肪酸发生了重组，从而使更高的膜完整性和精子活力的获得成为可能。然而，狗精子成熟过程中的脂肪酸分布仍然不清楚。本研究旨在鉴定精子成熟过程中附睾精子和血浆中的脂肪酸，并观察其运动性和质膜参数的变化。使用二十一只成年犬，进行双侧睾丸切除术和附睾保存，收集精子样本。对样品进行了常规显微镜检查，计算机辅助动力分析，精子质膜通透性和脂肪酸分析（脂质被提取，甲基化并通过色谱法进行了分析）。结果：Cap鱼和us体的精子值较低对于评估的运动性变量和质膜完整性，表明存在差异脂肪酸组织中的nt含量。附睾马尾精子中饱和，单不饱和和多不饱和脂肪酸的浓度较高。强调了辛酸，硬脂酸和二十二碳六烯酸的存在。精子成熟，使精子细胞发生物理和化学变化，对受精至关重要。

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《畜牧与生物技术杂志(英文版)》 |2017年第3期|574-581|共8页
作者
Daniel S.Ramos Angrimani; Marcilio Nichi; Joāo Diego A.Losano; Cristina F.Lucio; Gisele A.Lima Veiga; Márcia V.M.Junqueira Franco; Camila I.Vannucchi;
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Department of Animal Reproduction,School of Veterinary Medicine and Animal Science,University of S(ā)o Paulo,Rua Prof.Orlando Marques de Paiva,87,S(ā)o Paulo 05508-270,Brazil;

Department of Animal Reproduction,School of Veterinary Medicine and Animal Science,University of S(ā)o Paulo,Rua Prof.Orlando Marques de Paiva,87,S(ā)o Paulo 05508-270,Brazil;

Department of Animal Reproduction,School of Veterinary Medicine and Animal Science,University of S(ā)o Paulo,Rua Prof.Orlando Marques de Paiva,87,S(ā)o Paulo 05508-270,Brazil;

Department of Animal Reproduction,School of Veterinary Medicine and Animal Science,University of S(ā)o Paulo,Rua Prof.Orlando Marques de Paiva,87,S(ā)o Paulo 05508-270,Brazil;

Department of Animal Reproduction,School of Veterinary Medicine and Animal Science,University of S(ā)o Paulo,Rua Prof.Orlando Marques de Paiva,87,S(ā)o Paulo 05508-270,Brazil;

Department of Internal Medicine,Division of Nutrition,University of Sāo Paulo,Av.Bandeirantes,3900-14040-040,Ribeirāo Preto,Brazil;

Department of Animal Reproduction,School of Veterinary Medicine and Animal Science,University of S(ā)o Paulo,Rua Prof.Orlando Marques de Paiva,87,S(ā)o Paulo 05508-270,Brazil;

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Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs

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