Artificial rearing influences the morphology,permeability and redox state of the gastrointestinal tract of low and normal birth weight piglets

Hans Vergauwen; Chris Van Ginneken; Jeroen Degroote; Sara Prims; Wei Wang; Erik Fransen; Stefaan De Smet; Christophe Casteleyn; Steven Van Cruchten; Joris Michiels

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Artificial rearing influences the morphology,permeability and redox state of the gastrointestinal tract of low and normal birth weight piglets

机译：人工饲养会影响低体重和正常体重的仔猪胃肠道的形态，通透性和氧化还原状态

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Background:In this study the physiological implications of artificial rearing were investigated.Low (LBW) and 0normal birth weight (NBW) piglets were compared as they might react differently to stressors caused by artificial rearing.In total,42 pairs of LBW and NBW piglets from 16 litters suckled the sow until d1 9 of age or were artificially reared starting at d3 until d19 of age.Blood and tissue samples that were collected after euthanasia at 0,3,5,8 and 19 d of age.Histology,ELISA,and Ussing chamber analysis were used to study proximal and distal small intestine histomorphology,proliferation,apoptosis,tight junction protein expression,and permeability.Furthermore,small intestine,liver and systemic redox parameters (GSH,GSSG,GSH-Px and MDA) were investigated using HPLC.Results:LBW and NBW artificially reared piglets weighed respectively 40 and 33％ more than LBW and NBW sowreared piglets at d19 (P ＜ 0.01).Transferring piglets to a nursery at d3 resulted in villus atrophy,increased intestinal FD-4 and HRP permeability and elevated GSSG/GSH ratio in the distal small intestine at d5 (P ＜ 0.05).GSH concentrations in the proximal small intestine remained stable,while they decreased in the liver (P ＜ 0.05).From d5 until d 1 9,villus width and crypt depth increased,whereas PCNA,caspase-3,occludin and claudin-3 protein expressions were reduced.GSH,GSSG and permeability recovered in artificially reared piglets (P ＜ 0.05).Conclusion:The results suggest that artificial rearing altered the morphology,permeability and redox state without compromising piglet performance.The observed effects were not depending on birth weight.

机译：背景：本研究探讨了人工饲养的生理意义。比较了低体重（LBW）和0正常出生体重（NBW）的仔猪，因为它们对人工饲养引起的应激源的反应可能不同。总共42对LBW和NBW仔猪从16胎的母猪哺乳至d1 9龄，或从d3直到d19人工饲养。在安乐死后的0、3、5、8和19 d采集血液和组织样品。并利用Ussing chamber分析研究近端和远端小肠的组织形态，增殖，凋亡，紧密连接蛋白表达和通透性。此外，还研究了小肠，肝脏和全身氧化还原参数（GSH，GSSG，GSH-Px和MDA）结果：在第19天，LBW和NBW人工饲养的仔猪比LBW和NBW浸泡的仔猪分别重40％和33％（P ＜0.01）。在d3时将仔猪转移到育苗室会导致绒毛萎缩，并增加绒毛在d5时末梢小肠的FD-4和HRP通透性升高，GSSG / GSH比率升高（P ＜0.05）。在近端小肠中GSH的浓度保持稳定，而在肝脏中则下降（P ＜0.05）。直到d 1 9，绒毛宽度和隐窝深度增加，PCNA，caspase-3，occludin和claudin-3蛋白表达降低。人工饲养仔猪的GSH，GSSG和通透性恢复（P ＜0.05）。结论：结果提示人工饲养改变了形态，通透性和氧化还原状态而没有损害仔猪的生产性能。观察到的效果与出生体重无关。

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《畜牧与生物技术杂志(英文版)》 |2017年第3期|707-720|共14页
作者
Hans Vergauwen; Chris Van Ginneken; Jeroen Degroote; Sara Prims; Wei Wang; Erik Fransen; Stefaan De Smet; Christophe Casteleyn; Steven Van Cruchten; Joris Michiels;
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Laboratory of Applied Veterinary Morphology,Department of Veterinary Sciences,Faculty of Biomedical,Pharmaceutical and Veterinary Sciences,University of Antwerp,Campus Drie Eiken,Universiteitsplein 1,D.U.015,2610 Wilrijk,Belgium;

Laboratory of Applied Veterinary Morphology,Department of Veterinary Sciences,Faculty of Biomedical,Pharmaceutical and Veterinary Sciences,University of Antwerp,Campus Drie Eiken,Universiteitsplein 1,D.U.015,2610 Wilrijk,Belgium;

Department of Applied Biosciences,Faculty of Bioscience Engineering,Ghent University,Ghent,Belgium;

Laboratory of Applied Veterinary Morphology,Department of Veterinary Sciences,Faculty of Biomedical,Pharmaceutical and Veterinary Sciences,University of Antwerp,Campus Drie Eiken,Universiteitsplein 1,D.U.015,2610 Wilrijk,Belgium;

Department of Applied Biosciences,Faculty of Bioscience Engineering,Ghent University,Ghent,Belgium;

Laboratory for Animal Nutrition and Animal Product Quality(LANUPRO),Department of Animal Production,Faculty of Bioscience Engineering,Ghent University,Melle,Belgium;

StatUa Center for Statistics,University of Antwerp,Antwerp,Belgium;

Laboratory for Animal Nutrition and Animal Product Quality(LANUPRO),Department of Animal Production,Faculty of Bioscience Engineering,Ghent University,Melle,Belgium;

Laboratory of Applied Veterinary Morphology,Department of Veterinary Sciences,Faculty of Biomedical,Pharmaceutical and Veterinary Sciences,University of Antwerp,Campus Drie Eiken,Universiteitsplein 1,D.U.015,2610 Wilrijk,Belgium;

Laboratory of Applied Veterinary Morphology,Department of Veterinary Sciences,Faculty of Biomedical,Pharmaceutical and Veterinary Sciences,University of Antwerp,Campus Drie Eiken,Universiteitsplein 1,D.U.015,2610 Wilrijk,Belgium;

Department of Applied Biosciences,Faculty of Bioscience Engineering,Ghent University,Ghent,Belgium;

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Artificial rearing influences the morphology,permeability and redox state of the gastrointestinal tract of low and normal birth weight piglets

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