Expression of fatty acid sensing G-protein coupled receptors in peripartal Holstein COWS

Alea Agrawal; Abdulrahman Alharthi; Mario Vailati-Riboni; Zheng Zhou; Juan J.Loor

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Expression of fatty acid sensing G-protein coupled receptors in peripartal Holstein COWS

机译：脂肪酸敏感的G蛋白偶联受体在围产期荷斯坦奶牛中的表达

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Background:G-protein coupled receptors (GPCR),also referred as Free Fatty Acid Receptors (FFAR),are widely studied within human medicine as drug targets for metabolic disorders.To combat metabolic disorders prevalent in dairy cows during the transition period,which co-occur with negative energy balance and changes to lipid and glucose metabolism,it may be helpful to identify locations and roles of FFAR and other members of the GPCR family in bovine tissues.Results:Quantitative RT-PCR (qPCR) of subcutaneous adipose,liver,and PMNL samples during the transition period (-10,+7,and +20 or +30 d) were used for expression profiling of medium-(MCFA) and long-chain fatty acid (LCFA)receptors GPR120 and GPR40,MCFA receptor GPR84,and niacin receptor HCAR2/3.Adipose samples were obtained from cows with either high (HI;BCS ＞ 3.75) or low (LO;BCS ≤ 3.25) body condition score (BCS) to examine whether FFAR expression is correlated with this indicator of health and body reserves.Supplementation of rumen-protected methionine (MET),which may improve immune function and production postpartum,was also compared with unsupplemented control (CON) cows for liver and blood polymorphonuclear leukocytes (PMNL) samples.In adipose tissue,GPR84 and GPR120 were differentially expressed over time,while GPR40 was not expressed;in PMNL,GPR40 was differentially expressed over time and between MET vs.CON,GPR84 expression differed only between dietary groups,and GPR120 was not expressed;in liver,GPCR were either not expressed or barely detectable.Conclusions:The data indicate that there is likely not a direct role in liver for the selected GPCR during the transition period,but they do play variable roles in adipose and PMN.In future,these receptors may prove useful targets and/or markers for peripartal metabolism and immunity.

机译：背景：G蛋白偶联受体（GPCR），也称为游离脂肪酸受体（FFAR），在人类医学中被广泛研究为代谢紊乱的药物靶标。为了对抗过渡时期奶牛中普遍存在的代谢紊乱， -发生负能量平衡并改变脂质和葡萄糖代谢，可能有助于识别牛组织中FFAR和GPCR家族其他成员的位置和作用。结果：皮下脂肪，肝脏的定量RT-PCR（qPCR），并在过渡期（-10，+ 7和+20或+30 d）中将PMNL样品用于中型（MCFA）和长链脂肪酸（LCFA）受体GPR120和GPR40，MCFA受体的表达谱分析GPR84和烟酸受体HCAR2 / 3。从高（HI; BCS＞ 3.75）或低（LO; BCS≤3.25）身体状况评分（BCS）的母牛身上获取脂肪样本，以检查FFAR表达是否与此指标相关和身体储备的补充。瘤胃的补充与未补充对照（CON）的牛和肝脏多形核白细胞（PMNL）样品相比，可提高免疫功能和产后生产的n-保护的蛋氨酸（MET）。在脂肪组织中，GPR84和GPR120随时间而差异表达，但未表达GPR40;在PMNL中，GPR40随时间而差异表达，而MET与CON之间，GPR84表达仅在饮食组之间不同，而GPR120不表达;在肝脏中，GPCR不表达或几乎无法检测到。数据表明，在过渡期中所选的GPCR在肝脏中可能没有直接作用，但它们确实在脂肪和PMN中起可变作用。将来，这些受体可能被证明是围产期代谢的有用靶标和/或标志物和免疫力。

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《畜牧与生物技术杂志(英文版)》 |2017年第3期|660-669|共10页
作者
Alea Agrawal; Abdulrahman Alharthi; Mario Vailati-Riboni; Zheng Zhou; Juan J.Loor;
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Department of Animal Sciences and Division of Nutritional Sciences,University of Illinois,1207 West Gregory Drive,Urbana,IL 61801,USA;

Department of Animal Sciences and Division of Nutritional Sciences,University of Illinois,1207 West Gregory Drive,Urbana,IL 61801,USA;

Department of Animal Sciences and Division of Nutritional Sciences,University of Illinois,1207 West Gregory Drive,Urbana,IL 61801,USA;

Department of Animal Sciences and Division of Nutritional Sciences,University of Illinois,1207 West Gregory Drive,Urbana,IL 61801,USA;

Department of Animal Sciences and Division of Nutritional Sciences,University of Illinois,1207 West Gregory Drive,Urbana,IL 61801,USA;

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Expression of fatty acid sensing G-protein coupled receptors in peripartal Holstein COWS

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