The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet

Wei Jin; Yanfen Cheng; Weiyun Zhu

摘要

Background:The newly proposed methanogenic order'Methanomassiliicoccales' is the second largest archaeal population in the rumen,second only to the Methanobrevibacter population.However,information is limited regarding the community of this new order in the rumen.Methods:This study used real-time PCR and 454 pyrosequencing to explore the abundance and community composition of Methanomassiliicoccales in the rumen of Chinese goats fed a hay (0％ grain,n =5) or a high grain (65％ grain,n =5) diet.Results:Real-time PCR analysis showed that the relative abundance of Methanomassiliicoccales (％ of total archaea) in the goat rumen was significantly lower in the high-grain-diet group (0.5％ ± 0.2％) than that in the hay-diet group (8.2％ ± 1.1％,P ＜ 0.05).The pyrosequencing results showed that a total of 208 operational taxonomic units (OTUs) were formed from ten samples at 99％ sequence identity.All the sequences were identified as Methanomassiliicoccaceae at the family level,and most of the sequences (96.82％ ± 1.64％) were further classified as Group 8,9,and 10 at the Methanomasslliicoccales genus level in each sample based on the RIM-DB database.No significant differences were observed in the number of OTUs or Chao]'s,Shannon's or Pielou's evenness indexes between the hay-and high-grain-diet groups (P≥ 0.05).PCoA analysis showed that diet altered the community of Methanomassiliicoccales.At the genus level,the relative abundances of Group 10 (67.25 ± 12.76 vs.38.13 ± 15.66,P =0.012) and Group 4 (2.07 ± 1.30 vs.0.27 ± 0.30,P =0.035) were significantly higher in the high-grain-diet group,while the relative abundance of Group 9 was significantly higher in the hay-diet group (18.82 ± 6.20 vs.47.14 ± 17.72,P =0.020).At the species level,the relative abundance of Group 10 sp.(67.25 ± 12.76 vs.38.13 ± 15.66,P =0.012) and Group 4 sp.MpT1 (2.07 ± 1.30 vs.0.27 ± 0.30,P =0.035) were significantly higher in the high-grain-diet group,while the relative abundance of Group 9 sp.ISO4-G1 was significantly higher in the hay-diet group (12.83 ± 3.87 vs.42.44 ± 18.47,P =0.022).Conclusions:Only a few highly abundant phylogenetic groups dominated within the Methanomassiliicoccales community in the rumens of Chinese goats,and these were easily depressed by high-grain-diet feeding.The relatively low abundance suggests a small contribution on the part of Methanomassiliicoccales to the rumen methanogenesis of Chinese goats.

机译：背景：新提出的产甲烷菌“ Methanomassiliicoccales”是瘤胃中的第二大古细菌种群，仅次于甲基短杆菌属。然而，有关该新菌体在瘤胃中的信息有限。时间PCR和454焦磷酸测序，探讨饲喂干草（0％谷物，n = 5）或高谷物（65％谷物，n = 5）日粮的山羊山羊瘤胃中甲烷菌的丰度和群落组成。结果：真实实时PCR分析表明，高粒饮食组（占总古细菌的％）的甲烷甲烷相对丰度（0.5％±0.2％）明显低于干草饮食组（占8.2％）的相对丰度。 ±1.1％，P ＜0.05）。焦磷酸测序结果表明，从10个样品中以99％的序列同一性形成了总共208个操作分类单元（OTU）。在家庭水平上，所有序列均被鉴定为甲烷菌科。序列根据RIM-DB数据库，每个样品的甲烷菌属（Methanomasslliicoccales）属水平（96.82％±1.64％）进一步分为8,9和10组。在OTU或Chaos的数量上没有观察到显着差异。，干草和高粮饮食组之间的香农均匀度指数（P≥0.05）。PCoA分析显示，饮食改变了甲烷菌的种类。在属水平上，第10组的相对丰度（67.25±12.76 vs高谷物饮食组的饮食质量显着高于.38.13±15.66，P = 0.012）和第4组（2.07±1.30 vs.0.27±0.30，P = 0.035），而第9组的相对丰度则显着较高。干草饮食组（18.82±6.20 vs.47.14±17.72，P = 0.020）。在物种水平上，第10组的相对丰度（67.25±12.76 vs.38.13±15.66，P = 0.012）和第4组高粮饮食组MpT1（2.07±1.30 vs.0.27±0.30，P = 0.035）显着较高，而9组sp.ISO4-G1的相对丰度显着干草饮食组中高得多（12.83±3.87 vs.42.44±18.47，P = 0.022）。结论：在中国山羊瘤胃的甲烷单胞菌群落中只有少数高度丰富的系统发生组占主导地位，这些很容易被抑郁。相对较低的丰度表明，甲烷单胞菌对中国山羊瘤胃甲烷化的贡献很小。

The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet

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