Evidence of endometrial amino acid metabolism and transport modulation by peri-ovulatory endocrine profiles driving uterine receptivity

Moana Rodrigues Fran(c)a; Maressa Izabel Santos da Silva; Guilherme Pugliesi; Veerle Van Hoeck; Mario Binelli

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Evidence of endometrial amino acid metabolism and transport modulation by peri-ovulatory endocrine profiles driving uterine receptivity

机译：排卵周内分泌谱驱动子宫接受性的子宫内膜氨基酸代谢和转运调节的证据

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Background:In beef cattle,changes in the periovulatory endocrine milieu are associated with fertility and conceptus growth.A large preovulatory follicle (POF) and the resulting elevated concentrations of progesterone (P4) during diestrus positively affect pregnancy rates.Amino acids (AA) are important components of maternally derived secretions that are crucial for embryonic survival before implantation.The hypothesis is that the size of the POF and the concentration of P4 in early diestrus modulate the endometrial abundance of SLC transcripts related to AA transport and metabolism and subsequently impact luminal concentrations of AA.The follicle growth of Nelore cows was manipulated to produce two experimental groups:large POF and CL (LF-LCL group) and small POF and CL (SF-SCL group).On Day 4 (D4;Experiment 1) and Day 7 (D7;Experiment 2) after GnRH-induced ovulation (GnRH treatment=D0),the animals were slaughtered and uterine tissues and uterine washings were collected.qRT-PCR was used to evaluate the expression levels of AA transporters in D4 and D7 endometrial tissues.The concentrations of AA were quantified in D4 and D7 uterine washings by HPLC.Results:Transcript results show that,on D4,SLC6A6,SLC7A4,5LC17A5,SLC38A1,SLC38A7 and SCLY and on D7 SLC1A4,SLC6A1,SLC6A14,SLC7A4,SLC7A7,SLC7A8,SLC17A5,SLC38A1,SLC38AT,SLC43A2 and DDO were more abundant in the endometria of cows from the LF-LCL group (P ＜ 0.05).In addition,concentrations of AA in the uterine lumen were influenced by the endocrine profiles of the mother.In this context,D4 uterine washings revealed that greater concentrations of taurine,alanine and α-aminobutyric acid were present in SF-SCL (P ＜ 0.05).In contrast,lower concentrations of valine and cystathionine were quantified on D7 uterine washings from SF-SCL cows (P ＜ 0.05).Conclusion:The present study revealed an association between the abundance of transcripts related to AA transport and metabolism in the endometrium and specific periovulatory endocrine profiles related to the receptive status of the mother.Such insights suggest that AAs are involved in uterine function to support embryo development.

机译：背景：在肉牛中，排卵周内分泌环境的变化与生育能力和概念生长有关。排卵前大卵泡（POF）以及二孕期孕酮（P4）浓度升高对妊娠率产生积极影响。氨基酸（AA）是母体来源分泌物的重要组成部分对着床前的胚胎存活至关重要。这一假设是，早期雌激素中POF的大小和P4的浓度会调节与AA转运和代谢有关的SLC转录本的子宫内膜丰度，进而影响腔内浓度。第4天（D4;实验1）和第4天，操纵Nelore奶牛的卵泡生长产生两个实验组：大POF和CL（LF-LCL组）和小POF和CL（SF-SCL组）。 GnRH诱导排卵（GnRH处理= D0）后第7天（D7;实验2），将动物屠宰并收集子宫组织和子宫清洗液。使用qRT-PCR d评估D4和D7子宫内膜组织中AA转运蛋白的表达水平。通过HPLC定量测定D4和D7子宫清洗液中AA的浓度。在LF-LCL组的母牛的子宫内膜中，D7中SLC1A4，SLC6A1，SLC6A14，SLC7A4，SLC7A7，SLC7A8，SLC17A5，SLC38A1，SLC38AT，SLC43A2和DDO含量更高（P <0.05）。子宫内腔中AA的含量受母亲内分泌情况的影响。在这种情况下，D4子宫冲洗液显示SF-SCL中牛磺酸，丙氨酸和α-氨基丁酸的浓度较高（P ＜0.05）。 SF-SCL奶牛D7子宫冲洗液中的缬氨酸和胱硫醚含量较低（P ＜0.05）。与母亲的接受状态有关的资料。这些见解表明，AA参与子宫功能以支持胚胎发育。

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《畜牧与生物技术杂志(英文版)》 |2017年第4期|852-865|共14页
作者
Moana Rodrigues Fran(c)a; Maressa Izabel Santos da Silva; Guilherme Pugliesi; Veerle Van Hoeck; Mario Binelli;
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Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of S(a)o Paulo, 225, Duque de Caxias Norte Ave.Jd.Elite, 13635-900 Pirassununga, SP, Brazil;

Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of S(a)o Paulo, 225, Duque de Caxias Norte Ave.Jd.Elite, 13635-900 Pirassununga, SP, Brazil;

Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of S(a)o Paulo, 225, Duque de Caxias Norte Ave.Jd.Elite, 13635-900 Pirassununga, SP, Brazil;

Gamete Research Centre, University of Antwerp, Antwerp, Belgium;

Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of S(a)o Paulo, 225, Duque de Caxias Norte Ave.Jd.Elite, 13635-900 Pirassununga, SP, Brazil;

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Evidence of endometrial amino acid metabolism and transport modulation by peri-ovulatory endocrine profiles driving uterine receptivity

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