Effect of three lactobacilli with strain-specific activities on the growth performance, faecal microbiota and ileum mucosa proteomics of piglets

Yating Su; Xingjie Chen; Ming Liu; Xiaohua Guo

摘要

Background:The beneficial effects of Lactobacillus probiotics in animal production are often strain-related.Different strains from the same species may exert different weight-gain effect on hosts in vivo.Most lactobacilli are selected based on their in vitro activities,and their metabolism and regulation on the intestine based on strain-related characters are largely unexplored.The objective of the present study was to study the in vivo effects of the three lactobacilli on growth performance and to compare the differential effects of the strains on the faecal microbiota and ileum mucosa proteomics of piglets.Methods:Three hundred and sixty piglets were assigned to one of four treatments,which included an antibiotics-treated control and three experimental groups supplemented with the three lactobacilli,L.salivarius G1-1,L.reuteri G8-5 and L.reuteri G22-2,respectively.Piglets were weighed and the feed intake was recorded to compare the growth performance.The faecal lactobacilli and coliform was quantified using quantitative PCR and the faecal microbiota was profiled by denaturing gradient gel electrophoresis (DGGE).The proteomic approach was applied to compare the differential expression of proteins in the ileum mucosa.Results:No statistical difference was found among the three Lactobacillus-treated groups in animal growth performance compared with the antibiotics-treated group (P ＞ 0.05).Supplementation of lactobacilli in diets significantly increased the relative 16S rRNA gene copies of Lactobacillus genus on both d 14 and d 28 (P ＜ 0.05).,and the bacterial community profiles based on DGGE from the lactobacilli-treated groups were distinctly different from the antibiotics-treated group (P ＜ 0.05).The ileum mucosa of piglets responded to all Lactobacillus supplementation by producing more newly expressed proteins and the identified proteins were all associated with the functions beneficial for stabilization of cell structure.Besides,some other up-regulated and down-regulated proteins in different Lactobacillus-treated groups showed the expression of proteins were partly strain-related.Conclusions:All the three lactobacilli in this study show comparable effects to antibiotics on piglets growth performance.The three lactobacilli were found able to modify intestinal microbiota and mucosa proteomics.The regulation of protein expression in the intestinal mucosa are partly associated with the strains administrated in feed.

机译：背景：乳酸菌益生菌在动物生产中的有益作用通常与菌株有关。同一物种的不同菌株可能在体内对宿主产生不同的增重作用。大多数乳酸菌是根据它们的体外活性，代谢和代谢来选择的。本研究的目的是研究三种乳酸菌在体内对生长性能的影响，并比较该菌株对粪便微生物群和回肠粘膜的不同作用。方法：将360只仔猪分为四种处理方法之一，包括抗生素处理的对照组和三个实验组，分别补充三种乳酸菌，唾液乳杆菌G1-1，罗伊氏乳杆菌G8-5和乳杆菌。罗伊氏乳杆菌G22-2分别称重仔猪并记录采食量以比较其生长性能。粪便乳杆菌和大肠菌采用定量PCR定量分析orm，通过变性梯度凝胶电泳（DGGE）分析粪便中的微生物群。采用蛋白质组学方法比较回肠粘膜中蛋白质的差异表达。结果：三种乳酸菌之间无统计学差异。与抗生素治疗组相比，动物治疗组的动物生长性能更高（P＞ 0.05）。饮食中添加乳酸杆菌显着增加了乳酸菌属的相对16S rRNA基因拷贝在d 14和d 28时（P ＜0.05），并且乳杆菌治疗组基于DGGE的细菌群落特征与抗生素治疗组明显不同（P ＜0.05）。仔猪回肠粘膜对所有乳酸杆菌的补充有反应，产生更多新表达的蛋白，鉴定出的蛋白全部与有益于稳定细胞结构的功能有关。此外，还有其他一些上调和下调结论：本研究中的三种乳酸菌对仔猪的生长性能均具有与抗生素相当的作用。发现三种乳酸菌能够改变肠道菌群和黏膜。蛋白质组学。肠道粘膜中蛋白质表达的调节与饲料中施用的菌株部分相关。

Effect of three lactobacilli with strain-specific activities on the growth performance, faecal microbiota and ileum mucosa proteomics of piglets

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