目的：观察慢性噪声暴露后大鼠听皮层及海马脑区胰岛素样生长因子－1（insulin－like growth fac-tor－1，IGF－1）的表达，探讨其在长期噪声性中枢神经系统损伤中的作用。方法成年健康雄性 Wistar 大鼠16只，随机分为噪声组和对照组各8只，噪声组暴露于100 dB SPL 白噪声28天，每天4小时，制成慢性噪声暴露模型，对照组不予任何处理。造模结束后检测两组大鼠 ABR 反应阈，并采用免疫组织化学染色方法检测 IGF－1在听皮层和海马的表达。结果噪声组造模结束后 ABR 反应阈（80．62±4．58 dB SPL）较对照组（38．75±3．54 dB SPL）明显升高（P＜0．05），听皮层及海马脑区 IGF－1阳性神经元数目和表达强度均较对照组显著增加（P＜0．05）。结论慢性噪声暴露可以使听皮层及边缘系统海马脑区 IGF－1表达增高，这可能与其对中枢神经系统噪声性损伤的保护作用有关。%Objective To observe the expression of the IGF-1 on the auditory cortex as well as the hippo-campus of rats which underwent longterm exposure to white noise to explore its effects for the repairment of the noise induced damage in the central nervous system.Methods 16 healthy Wistar rats were randomly grouped as chronic noise exposure group(group A)which underwent the longterm noise exposure(100 dB SPL white noise,4 hours per day for 28 days)and control group(group B).The expression of IGF-1 both on the auditory cortex and hippocampus was measured and the ABR waveforms were recorded.ResuIts Compared with the group A,the num-ber of IGF-1 positive neurons as well as the expression of IGF-1 both in the auditory cortex and the hippocampus of the group B increased(P<0.05),the ABR threshold was significantly higher(P<0.05 )after long-term noise exposure.ConcIusion Chronic noise exposure induced the changes of the IGF-1 system which may play a part in the protection for the noise-induced damage of the central nervous system.
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