针对目前检测脱氧雪腐镰刀菌烯醇( DON )的常用方法在检出时间或者灵敏度上都存在不足的问题,研究制备出了羧基修饰的核壳结构上转换荧光纳米颗粒,并与DON单克隆抗体偶联,应用于试纸对DON进行定量检测. 对麦片中DON含量的最低检测限为0. 7 ng/mL. 半抑制浓度为9 ng/mL,检测范围为0. 7～50. 0 ng/mL, 回归率在90% ～156%. 本研究基于上转换免疫层析技术成功建立了一种快速灵敏的检测DON的方法,可为其他包括毒素类的兽药残留检测提供借鉴.%The common methods of detection deoxynivalenol ( DON ) are limited to time-consuming or lower sensitivity. The carboxyl modification of core/shell structure up-converting nanoparticles was produced and conjugated to anti-DON antibodies, and then applied in lateral flow strip to detection DON. The detection of limitation in cornmeal was 0. 7 ng/mL. The 50% of inhibit concentration was 9 ng/mL. The detection range was from 0. 7 to 50 ng/mL and the recovery rates were from 90% to 156 %. The study has developed method for detecting DON rapidly and sensitively based on up-converting immunochromatographic technology, which can also provided reference to the detection of veterinary residues.
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