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首页> 中文期刊> 《北京中医药大学学报》 >不同频率电针及刺激次数对心肌缺血大鼠内关穴与非经非穴点皮肤血流灌注量的影响

不同频率电针及刺激次数对心肌缺血大鼠内关穴与非经非穴点皮肤血流灌注量的影响

         
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目的：借助大鼠心肌缺血损伤模型，探讨低频或高频电针刺激内关穴后内关穴及非经非穴皮肤血流灌注量的变化规律及治疗次数对其的影响。方法将100只雄性 Wistar 大鼠按照随机数字表法分为1次治疗组和3次治疗组，各组再分为正常组、假手术组、模型组、低频电针组、高频电针组5个小组，每组10只。电针组大鼠左侧内关穴每日接受针刺1次，于第1天或第3天治疗结束60 min 后，应用激光散斑衬比成像技术检测各组大鼠双侧内关穴和非经非穴区域的皮肤血流灌注量，HE 染色观测心肌组织形态学变化。结果与正常组相比较，3次治疗后模型组内关穴区皮肤血流量显著降低（P ＜0．01），且3次治疗模型组较1次治疗模型组内关和非经非穴皮肤血流量皆显著降低（P ＜0．01）；与模型组相比较，3次治疗后低频电针组和高频电针组内关穴区皮肤血流量均显著升高（P ＜0．01）。结论内关穴区皮肤血流灌注量较非经非穴能相对特异地反映大鼠心肌处于不同状态的变化，尤其是低频或高频治疗后随次数增加能阻抑由于心肌缺血损伤诱发的相关经穴皮肤血流灌注量下降的情况，且3次治疗后以高频电针作用更为明显，其机制有待进一步研究。%Objective To compare the skin blood perfusion around Neiguan (PC 6)and sham acupoint needled by the different frequency and stimulation times of electro-acupuncture (EA),in healthy rats and myocardial ischemic rats.Methods According to random number table,altogether 100 male Wistar rats were divided into once treatment group and three times treatment group,which were both divided into control group,sham-operation group,model group,EA low-frequency group and EA high-frequency group (each n =10).After successful modeling,rats of EA groups were acupunctured at left Neiguan (PC 6)acupoint.60 minutes after last treatment of acupuncture,skin blood perfusion around bilateral Neiguan(PC 6)acapoints and sham acupoint were tested by laser speckle contrast imaging technology (LSCI) and analyzed,and the myocardial morphological changes were observed by HE staining. Results Compared with control group,the skin blood perfusion around PC 6 in model groups decreased significantly after three time treatment (P <0.01 ),and that around PC 6 or sham acupoint in model group of three times treatment group was much less than that of once treatment group (P <0.01).Com-pared with the model group,the skin blood perfusion around PC 6 both significantly increased after low-frequency EA or high-frequency EA after three times treatment (P <0.01).Conclusion The variation of skin blood perfusion around PC 6 should be useful for specific reflection of normal condition or ische-mic condition of rats myocardium,but that around sham acupoint didn’t.The effect of three-times-treat-ment against declination of skin blood perfusion due to myocardial ischemia was superior to that of once-treatment regardless of high frequency or low frequency,and EA of high frequency and three-times stimu-lation showed the significant effect.However,the mechanism remains to be further studied.

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