Objective: To interpret the possihle anti-tumor mechanism of adenovirus-mediated inhibitor of growth family member 4 ( Ad-ING4) by testing the growth and vascular endothelial growth factor( VEGF) expression of ECV304 cells infected by Ad-lNG4. Methods : Adenovirus vector of ING4 was constructed, then the growth of ECV304 cells was detected by MTT, and the VEGF expression of ECV304 cells infected by Ad-ING4 was detected by ELISA. Results : The VEGF expression of ECV304 cells infected by Ad-ING4 decreased, and the growth of ECV304 was inhihited, and the inhibition ratio was 63. 6％ on the fourth day. Conclusions : ING4 could down regulate the expression of VEGF , and inhibit the growth of ECV304. and ING4 maybe through this way affect growth, and tumor angiogenesis of lung cancer.%目的:检测腺病毒介导的生长抑制基因4(adenovirus-mediated inhibitor of growth family member 4,Ad-ING4)感染后ECV304细胞体外生长及分泌血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响,探讨ING4抑制肿瘤生长的可能机制.方法:构建ING4腺病毒载体,用MTT法检测Ad-ING4感染后ECV304细胞体外生长影响,ELISA检测VEGF的分泌情况.结果:Ad-ING4感染后第3天和第4天ECV304细胞体外生长均受到抑制(P<0.01),第4天时抑制率达63.6%,VEGF分泌受到抑制.结论:ING4能抑制ECV304细胞生长及VEGF分泌.Ad-ING4能抑制血管内皮细胞生长和血管的生成,从而可抑制体内肿瘤的生长.
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