分别以AFP多肽、HSP70 和HSP70-AFP多肽复合物免疫小鼠,检测各组CD8+T细胞培养上清IFN-γ、TNF-α、穿孔素、颗粒酶B水平及CD8+T细胞对H22细胞的杀伤作用,探讨热休克蛋白70(HSP70)-甲胎蛋白(AFP)抗原表位肽复合物对小鼠H22肝癌细胞的免疫效应.%To study anti-tumor immunity mechanism induced by a reconstructed peptide complex-heat shock protein 70 (HSP70) and alpha-fetoprotein (AFP), mice were immunized with recombinant peptide vaccine HSP70-AFP-P, whereas AFP peptide or HSP70 vaccination as controls.IFN-γ, TNF-α and granzyme-B, perforin-produced by separated CD8+ T cells from vaccinated mice were detected with ELISA, respectively.In vivo tumor loaded test was exerted to evaluate the immune effect of the peptide vaccine.The study suggested that sequential immunization with HSP70-AFP peptide vaccine could bring about effective AFP-specific CD8+ T cell responses and elicit specific antitumor immunity on AFP-producing tumors.
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