Aberrant glycosylation is known to be closely associated with cancer progression, and glycans are synthesized by glycosyl-transferases.Therefore,we studied the effect of β4GalT1 on cell behavior in bladder cancer.In this study,human HCV29 non-malig-nant urothelial cells and YTS1 bladder cancer cells were used as the cell models.The terminal lactosyl epitopes were elevated in YTS 1 by lectin staining and the expression of β4GalT1 was found up-regulated in YTS1 compared with HCV29 using western blot and Real-time-PCR test.In TGF-βinduced EMT process, the expression of β4GalT1 and terminal lactosyl epitopes significantly increased in TGF-β-treated HCV29.The cell migration increased in β4GalT1 overexpressed HCV29,and the cell migration decreased in β4GalT1 knockdown HCV29,which demonstrated that β4GalT1 could promote the migration of bladder cells.Further research is required to un-derstand the effect of β4GalT1 on the cancer progression.%在肿瘤发生发展过程中常伴随着糖基化的异常,而糖类结构是糖基转移酶的催化所形成,所以以膀胱癌细胞株为模型研究β1,4-半乳糖基转移酶1(β1,4-galactosyltransferase 1,β4GalT1)对细胞行为的影响.以人源正常膀胱上皮细胞HCV29和人源浸润性膀胱癌细胞YTS1为研究对象,通过凝集素荧光染色技术检测发现YTS1中末端乳糖胺结构含量升高,蛋白免疫印迹和实时荧光定量PCR技术检测发现YTS1中β4GalT1的表达显著高于HCV29.利用转化生长因子(transforming growth factor beta,TGF-β)诱导HCV29发生上皮间质转化(epithelial-mesenchymal transition,EMT)过程,经检测发现,末端乳糖胺结构与β4GalT1的表达显著升高.HCV29过表达β4GalT1后,细胞迁移速度明显变快,而干扰YTS1细胞中的β4GalT1后,细胞迁移速度明显变慢.由此表明,β4GalT1能促进膀胱细胞的迁移能力,但其对膀胱癌发生发展的影响有待深入研究.
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