磁小体( Magnetosome)是一种由趋磁细菌( Magnetotactic bacteria)产生的磁性纳米颗粒,具有生物相容性好、大小均一及超顺磁性等特点,已在免疫检测、载药体系、固定化酶等多领域得到广泛应用,但在糖蛋白分离纯化方面的研究还较少. 实验利用磷脂酰乙醇胺( PE)修饰磁小体,以双(硫代琥珀酰亚胺基)辛二酸酯钠盐( BS3 )为连接臂,制备凝集素-PE-磁小体复合物,用于糖蛋白的分离. 实验结果表明,改造后的PE-磁小体分散性更好,每毫克PE-磁小体可偶联294. 64 μg 麦胚凝集素( WGA) ,较修饰前提高1. 6倍. 在外加磁场作用下,所制备的WGA-PE-磁小体可以特异性的从鸡卵清蛋白( OVA)及伴刀豆凝集素( ConA)混合液中分离OVA. 因此,制备的凝集素-PE-磁小体复合物能很好的应用于糖蛋白分离纯化,从而在糖组学研究中发挥重要作用.%Magnetosomes synthesized by magnetotactic bacteria were drawn great interest as potential carriers to couple with diverse bioactive macromolecules, anticancer drugs and enzymes due to their unique features such as paramagnetism, nano-scale, narrow size distribution and membrane-bounded. However, it was rarely mentioned on the separation of glycoproteins using magnetosomes. In this paper, the magnetosomes were extracted from Magnetospirillum gryphiswaldense MSR-1, modified with phosphatidylethanolamine ( PE) , coupled with Bis ( sulfoduccinimidyl) suberate sodium salt ( BS3 ) , and ligated with lectin, sequentially. Lectin-PE-magneto-some complexes were prepared and used for the separation of glycoproteins. The results showed that the proteins on magnetosomes sur-face were removed, and modified PE-magnetosomes showed the better dispersion than native magnetosomes. Moreover, 294. 64 μg of wheat germ agglutinin ( WGA) were coupled to one milligram of PE-magnetosomes, 1. 6 times than that coupled to one milligram of magnetosomes. WGA-PE-magnetosomes could specifically separate the ovalbumin ( OVA) from the mixture of OVA and concanavalin A ( ConA) . In all, it has the great potential application in separation and enrichment of glycoproteins and used in the glycomics.
展开▼
机译：预防和治疗上皮伤口的药物组合物，免疫调节药物组合物，药物组合物，杀虫剂，杀虫剂组合物杀虫剂使用凝集素KM +来治疗结疤，使用凝集素KM +来制备免疫调节药物，使用凝集素KM +来制备抗菌药物，用途lectin KM +用于制备抗病毒药物，使用lectin KM +制备抗寄生虫药物，使用lectin KM +制备抗真菌药物，使用lectin KM +制备抗寄生虫药物表达方法，DNA载体，重组体
