类芋螺毒素(CTX)基因存在于多种杆状病毒,编码富含半胱氨酸的多肽.从AcMNPV中克隆了ctx基因,利用AcMNPV杆粒操作系统构建了重组病毒Ac-pFastBac1-Pie1-ctx-eGFP和Ac-pFastBacl-Pie1-ctx.绿色荧光蛋白介导的亚细胞定位显示AcMNPV CTX定位于Tn细胞的细胞膜；抑菌实验表明,重组病毒Ac-pFastBacl-Piel-ctx感染的胞外产物对金黄色葡萄球菌、微球菌、短小芽孢杆菌等临床致病菌具有显著的抗菌活性,但其作用机制尚需进一步阐明.%Conotoxin-like (CTX) gene encodes a small cysteine-rich peptide in various baculoviruses. In this study, the ctx gene was cloned from AcMNPV, and the recombinant AcMNPVs (Ac-pFastBac1-Pie1-ctx-eGFP and Ac-pFastBac1-Pie1-ctx) was generated using AcMNPV bacmid system. Green fluorescence in CTX-eGFP-positive Tn cells was mainly observed on the cell membrane. The results also indicated that extracullular products of Tn cells infected by Ac-pFastBac1-Pie1-ctx had antimicrobial activity to some clinical pathogenic bactera (Staphylococcus aureus, Micrococcus and Bacillus pumilus).
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