FGF-2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles

Arzu; Hizay; Mark; Seitz; Maria; Grosheva; Nektarios; Sinis; Yasemin; Kaya; Habib; Bendella; Levent; Sarikcioglu; Sarah; A.Dunlop; Doychin; N.Angelov

首页> 中文期刊> 《生物医学研究杂志：英文版》 >FGF-2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles

FGF-2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles

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Recently,we have shown that manual stimulation of paralyzed vibrissal muscles after facial-facial anastomosis reduced the poly-innervation of neuromuscular junctions and restored vibrissal whisking.Using gene knock outs,we found a differential dependence of manual stimulation effects on growth factors.Thus,insulin-like growth factor-1 and brain-derived neurotrophic factor are required to underpin manual stimulation-mediated improvements,whereas FGF-2 is not.The lack of dependence on FGF-2 in mediating these peripheral effects prompted us to look centrally,i.e.within the facial nucleus where increased astrogliosis after facial-facial anastomosis follows "synaptic stripping".We measured the intensity of Cy3-fluorescence after immunostaining for glial fibrillary acidic protein(GFAP) as an indirect indicator of synaptic coverage of axotomized neurons in the facial nucleus of mice lacking FGF-2(FGF-2^(-/-) mice).There was no difference in GFAP-Cy3-fluorescence(pixel number,gray value range17-103) between intact wildtype mice(2.12± 0.37×10~7) and their intact FGF-2^(-/-) counterparts(2.12±0.27×10~7) nor after facial-facial anastomosis +handling(wildtype:4.06±0.32×10~7;FGF-2^(-/-):4.39±0.17×10~7).However,after facial-facial anastomosis,GFAP-Cy3-fluorescence remained elevated in FGF-2^(-/-)-animals(4.54±0.12×10~7),whereas manual otimulation reduced the intensity of GFAP-immunofluorescence in wild type mice to values that were not significantly different from intact mice(2.63±0.39×10).We conclude that FGF-2 is not required to underpin the beneficial effects of manual stimulation at the neuro-muscular junction,but it is required to minimize astrogliosis in the brainstem and,by implication,restore synaptic coverage of recovering facial motoneurons.

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《生物医学研究杂志：英文版》 |2016年第002期|P.142-148|共7页
作者
Arzu; Hizay; Mark; Seitz; Maria; Grosheva; Nektarios; Sinis; Yasemin; Kaya; Habib; Bendella; Levent; Sarikcioglu; Sarah; A.Dunlop; Doychin; N.Angelov;
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作者单位

[1]Department of Anatomy, Akdeniz University, Faculty of Medicine, Dumlupinar Bulvari 07058 Kampus, Antalya, Turkey,;

[2]Department of Anatomy I, University of Cologne, Joseph-Stelzmann-Strasse 9, D-50924 Cologne, FR Germany,;

[3]Department of Oto-Rhino-Laryngology, University Hospital Cologne, Joseph-Stelzmann-Strasse 9, D-50924 Cologne,FR Germany,;

[4]Depeartment of Plastic-, Hand-andReconstructive Microsurgery, Berlin, FR 12249, Germany,;

[5]Department of Neurosurgery, Hospital Merheim, University of Witten-Herdecke, Ostmerheimer StraJ3e 200, 51109 Cologne, Germany,;

[6]Experimental and Regenerative Neurosciences, School of Animal Biology, University of Western Australia （M092） 35Stirling Highway, CRA WLEY WA 6009, Australia.;

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原文格式 PDF
正文语种 CHI
中图分类 Q959.53;
关键词
胶质纤维酸性蛋白; 神经肌肉接头; 面神经核; 细胞增生; 神经损伤; 星形; 脑源性神经营养因子; 运动神经元;

机译：胶质纤维酸性蛋白;神经肌肉接头;面神经核;细胞增生;神经损伤;星形;脑源性神经营养因子;运动神经元;

FGF-2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles

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