Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine

Ali Ahadi; Alireza Partoazar; Mohammad-Hassan Abedi-Khorasgani; Seyed Vahid Shetab-Boushehri

首页> 中文期刊> 《生物医学研究杂志（英文版）》 >Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine

Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine

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Liquid-liquid extraction-thin layer chromatography (LLE-TLC) has been a common and routine combined method for detection of drugs in biological materials.Solid-phase extraction (SPE) is gradually replacing the traditional LLE method.High performance thin layer chromatography (HPTLC) has several advantages over TLC.The present work studied the higher efficiency of a new SPE-HPTLC method over that of a routine LLE-TLC method,in extraction and detection of urinary morphine.Fifty-eight urine samples,primarily identified as morphine-positive samples by a strip test,were re-screened by LLE-TLC and SPE-HPTLC.The results of LLE-TLC and SPE-HPTLC were then compared with each other.The results showed that the SPE-HPTLC detected 74% of total samples as morphine-positive samples whereas the LLE-TLC detected 48% of the same samples.We further discussed the effect of codeine abuse on TLC analysis of urinary morphine.Regarding the importance of morphine detection in urine,the present combined SPE-HPTLC method is suggested as a replacement method for detection of urinary morphine by many reference laboratories.

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《生物医学研究杂志（英文版）》 |2011年第5期|362-367|共6页
作者
Ali Ahadi; Alireza Partoazar; Mohammad-Hassan Abedi-Khorasgani; Seyed Vahid Shetab-Boushehri;
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Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, I.R. 1449614535 Iran;

Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, I.R. 1449614535 Iran;

Legal Medicine Organization of Iran, Tehran, I. R.1449614535, Iran;

Department of Medical Nanotechnology, School of Medicine, Tehran University of Medical Sciences, Tehran, I.R. 1449614535 Iran;

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Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine

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