Objective To observe the neuroprotection of 17β-estradiol on neurons and effects on expression of Brain Derived Neurotrophic Factor in cerebral cortex of rat after forebrain ischemia reperfusion. Methods 40 ovariectomized female SD rats were divided into four groups randomdy: saline + sham; 17β-estradiol + sham; 17β-estradiol + forebrain ischemia; saline + forebrain ischemia. Forebrain ischemia was induced by bilateral occlusion of the common caroid arteries for ten minutes combined with hypotension. The sham only uncovered the common arteries. Rats were treated with 17β-estradiol or saline by subabdnomimal injection for four weeks. 24 hours after forebrain ischemia or sham the rat was killed, brain was removed and paraffin-embedded. Sectioned for HE and BDNF stain, countering the number of necrosis and BDNF immunopositive neurons, T test and two-way classification ANOVA were used to analysis. Results 17β-estradiol could abviously reduce the number of necrosis neurons and increase the number of BDNF immunopositive neurons in cerebal cortex of rat ( not consider the effect of ischemia reperfusion,all p ＜ 0.01 ). Conclusion 17β-estradiol can upregulate the expression of BDNF in cerebral cortex of rat after forebrain ischemia reperfusion, which may be one of the nueroprotection mechanism of 17β-estradiol.%目的 观察17β-雌二醇对前脑缺血-再灌注大鼠大脑皮层神经元保护作用及脑源性神经生长因子(BDNF)表达的影响.方法 双侧卵巢切除的雌性SD大鼠随机分为四组:生理盐水+假手术组;17β-雌二醇+假手术组;17β-雌二醇+短暂性前脑缺血模型组;生理盐水+短暂性前脑缺血模型组.以低血压联合双侧颈总动脉夹闭10min的方法造前脑缺血模型,只暴露双侧颈总动脉为假手术,17β-雌二醇为药物干预,生理盐水为安慰剂,腹腔注射应用4周.在造模术或假手术后24h处死大鼠,用HE染色检测大脑皮层坏死神经元数目,免疫组织化学染色检测皮层BDNF免疫阳性细胞数,用t检验和两因素析因分析进行统计分析.结果 17β-雌二醇能显著减少大鼠大脑皮层坏死神经元数目,增加BDNF免疫阳性细胞数(p<0.01).结论17β-雌二醇对大鼠大脑皮层的神经神经保护作用可能是通过增加相应部位BDNF的表达来实现的.
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