目的:比较人骨髓、脂肪和脐带来源的间充质干细胞(mesenchymal stem cells,MSCs)促进内皮祖细胞(endothelial progenitor cells,EPCs)形成血管以及维持血管稳定的能力.方法:采用体外共培养成血管试验比较3种来源的MSCs促EPCs形成管状结构的能力;采用体内基质胶Matrigel成血管试验、免疫组织化学染色比较3种MSCs促进EPCs在体内形成功能血管的能力.结果:EPCs在脂肪MSCs单层上形成的管状结构长度和节点数均高于在骨髓和脐带MSCs单层上的形成数;EPCs与脂肪MSCs在Matrigel上共培养时形成的毛细血管样结构稳定性高于骨髓和脐带MSCs;脂肪MSCs与EPCs在体内Matrigel中形成大量有血流灌注的功能血管,脐带MSCs与EPCs在体内Matrigel中形成少量有血流灌注的功能血管,而骨髓MSCs与EPCs在体内Matrigel中只形成有血细胞渗漏的不完整血管.结论:脂肪MSCs在体内、体外促EPCs形成血管结构并维持其稳定的能力高于骨髓和脐带MSCs.%Objective:To compare the ability between bone marrow-derived mesenchymal stem cells (MSCs) (BM-MSCs) and adipose-derived MSCs (AD-MSCs) or umbilical cord-derived MSCs (UC-MSCs) on promotion of vessels formation and vessds stabilization relevant to the functions of EPCs.Methods:In vitro,co-culture blood vessel test was performed to compare the angiogenic ability between BM-MSCs,AD-MSCs or UC-MSCs.In vivo,angiogenic assay dependent on basement membrane matrix Matrigel and immunohistochemistry were performed to compare the ability of vessels formation functions between BM-MSCs and AD-MSCs or UC-MSCs.Results:The lengths and dots of vascular structures formed by EPCs on AD-MSCs layer are greater than those by EPCs on BM-MSCs layer and UC-MSCs layer in angiogenic assay in vitro.The stability of the capillary-like structures formed by EPCs with AD-MSCs on Matrigel was more stable than that by the BM-MSCs,UC-MSCs or EPCs.AD-MSCs and EPCs could form abundant functional vessels with blood perfusion in Matrigelin vivo;UC-MSCs and EPCs could form a few functional vessels with blood perfusion in Matrigelin vivo;BM-MSCs and EPCs could form broken vessels with hemocytes leakage in Matrigel in vivo.Conclusion:AD-MSCs have the stronger ability to promote the angiogenesis and stabilize the vessels compared with BM-MSCs or UC-MSCs ex vivo and in vivo.
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