Full-automatic rapid detector of food safety generally requires that only when the tested samples and reagents react at a spe-cific temperature condition, can reliable results be obtained.This paper designs a microcontroller-based constant temperature control system, which chooses microcontroller 89c52 as the controller, and ADS1256 chip, platinum resistance Pt100, optical coupler isolator and the third-generation Power MOSFETS device IRF640N as the hardware of circuits.After laboratory tests, the temperature control system of this design can control the temperature of the reaction solution in the cuvette within ±0.2℃and the temperature fluctuation is within ±0.1℃, it also has a reference value to the relevant issues and application.%全自动食品安全快速检测仪一般要求被检测样品和试剂只有在特定的温度下反应，才能得到可靠的检测结果，因此本文设计了一种基单片机的恒温控制系统。该系统选用89c52单片机为控制器，ADS1256芯片、铂电阻Pt100、光耦隔离器和第三代Power MOSFETS器件IRF640N作为电路的硬件。经过实验测试，本文设计的恒温控制系统能够控制比色杯内的反应液温度精确控制在±0．2℃波动范围内，而测试间温度波动在±0．1℃内，对相关课题的研究与应用具有一定的实用和参考价值。
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